Name | Herr Prof. Dr. Randall Platt |
Lehrgebiet | Biologisches Engineering |
Adresse | Professur Biologisches Engineering ETH Zürich, D-BSSE, BSA M 754 Mattenstrasse 26 4058 Basel SWITZERLAND |
Telefon | +41 61 387 33 50 |
randall.platt@bsse.ethz.ch | |
Departement | Biosysteme |
Beziehung | Ausserordentlicher Professor |
Nummer | Titel | ECTS | Umfang | Dozierende | |
---|---|---|---|---|---|
636-0113-00L | Genome Engineering | 4 KP | 3V | R. Platt | |
Kurzbeschreibung | This course is an introduction to genome engineering and an examination of recent advancements and future challenges. It covers the discovery and development of gene editing technologies and their applications in basic and applied research. The focus is on gaining an in-depth molecular and cellular understanding of the technologies and also insight into how these tools can be used and further deve | ||||
Lernziel | The objective of this course is to learn how gene editing technologies function at the molecular and cellular level, and how they are leveraged to understand the role of genetic elements in biological processes. Students will be introduced to the history and motivation behind the discovery and development of transformative genome engineering technologies, and also gain insight into the ethical, safety, and regulatory facets shaping the field. | ||||
Inhalt | The course content is comprised of lectures, discussions of important literature in the field, and a project. Lectures in Genome Engineering will be technology-focused and incorporate: 1) historical context to motivate the need for developing the technology, 2) development of the technology from concept to robust tool, 3) methods to discover, characterize, and evaluate the technology, and 4) applications of the technology in basic and applied research. Discussions of important literature in the field will be conducted in class, one course meeting following a lecture covering the topic material and assignment of the reading. The project will be team-based and entail devising a solution to a critical need in the field. Main topics: --Discovery and development of genome editing technologies --The prokaryotic adaptive immune system CRISPR-Cas --Genome engineering methods for generating genetically engineered model systems --Genotype-phenotype linkage via genetic screens --Massively paralleled perturbation and phenotyping --Gene editing tools as genetic recording devices --Gene editing tools as diagnostics and therapeutics --Ethics, safety, and regulatory facets of genome engineering | ||||
Skript | Handout during the course. | ||||
Literatur | Peer-reviewed scientific publications will be assigned to complement lecture content. | ||||
636-0301-00L | Current Topics in Biosystems Science and Engineering | 2 KP | 1S | T. Stadler, N. Beerenwinkel, Y. Benenson, K. M. Borgwardt, P. S. Dittrich, M. Fussenegger, A. Hierlemann, D. Iber, M. H. Khammash, D. J. Müller, S. Panke, R. Paro, R. Platt, S. Reddy, T. Schroeder, J. Stelling | |
Kurzbeschreibung | This seminar will feature invited lectures about recent advances and developments in systems biology, including topics from biology, bioengineering, and computational biology. | ||||
Lernziel | To provide an overview of current systems biology research. | ||||
Inhalt | The final list of topics will be available at http://www.bsse.ethz.ch/education/. | ||||
636-1004-AAL | Bio IV: Genetics Belegung ist NUR erlaubt für MSc Studierende, die diese Lerneinheit als Auflagenfach verfügt haben. Alle anderen Studierenden (u.a. auch Mobilitätsstudierende, Doktorierende) können diese Lerneinheit NICHT belegen. | 5 KP | 7R | R. Platt | |
Kurzbeschreibung | |||||
Lernziel | |||||
Literatur | Lewin, Genes XI, chapters: 3,15, 16, 19-23, 26-30 | ||||
636-1008-AAL | Bio Lab III: Molecular Biology I Belegung ist NUR erlaubt für MSc Studierende, die diese Lerneinheit als Auflagenfach verfügt haben. Alle anderen Studierenden (u.a. auch Mobilitätsstudierende, Doktorierende) können diese Lerneinheit NICHT belegen. | 1 KP | 3R | R. Platt | |
Kurzbeschreibung | |||||
Lernziel | |||||
Inhalt | PCR, restriction digest, ligation, transformation, gel analysis, Sanger sequencing, Gibson assembly |