Christoph Hartmut Giese: Catalogue data in Spring Semester 2023 |
Name | Dr. Christoph Hartmut Giese |
Address | Inst. f. Molekularbiol.u.Biophysik ETH Zürich, HPK E 2 Otto-Stern-Weg 5 8093 Zürich SWITZERLAND |
Telephone | +41 44 633 32 92 |
giesec@mol.biol.ethz.ch | |
Department | Biology |
Relationship | Lecturer |
Number | Title | ECTS | Hours | Lecturers | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
529-0074-00L | BCB IV: Analytical Biochemistry and Biophysics | 5 credits | 7P | K. Lang, J. W. Bode, M. Fottner, C. H. Giese, E. C. Meister, T. Segawa | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Abstract | Practical Introduction to Experimental Methods in Analytical and Bioanalytical Chemistry | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Learning objective | To acquire a sound knowledge of the instrumentation and methods for the analysis of molecules, including organic compounds and biological molecules. To learnabout the most important practical techniques in analytical chemistry. To write critical reports for each experiment and analytical technique. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Content | The course will comprise of maximum 10 experiments from the following themes: Spectroscopic structural characterisation, analytical characterisation, Bio-physical characterisation. Experiments that will be part of this curriculum are: Circular dichroism (CD) spectroscopy, UV spectroscopy, Mass Spectromety, Nuclear Magnetic Resonance, HPLC and Size exclusion separations, Isothermal titration calorimetry as well as a number of other bio-physical and biological characterisation techniques. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lecture notes | Learning Materials will be made available on an online platform (web page or Moodle) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Literature | Learning Materials will be made available on an online platform (web page or Moodle) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Prerequisites / Notice | Prerequisites / Notice This practical course is only for students enrolled in BCB. Additionally the following courses have to be successfully completed: 529-0016-00 P BCBIII Organic Chemistry (Praktikum) 529-0011-04 "Allgemeine Chemie (Praktikum)" 529-0051-00 "Analytische Chemie" I (3. Semester) 551-0128-00L Fundamentals of Biology I Furthermore, enrolment in parallel to the lab class, or completed in an earlier semester is requested for the following course: 529-0058-00 "Analytische Chemie II" (4. Semester) BCB Students should also be enrolled in "Instumentalanalyse organischer Verbindungen". | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Competencies |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
551-0128-00L | Fundamentals of Biology I Registrations via myStudies until 28.1.2023 at the latest. Subsequent registrations will not be considered. | 8 credits | 8P | M. Gstaiger, A. Cléry, E. Dultz, C. H. Giese, R. Kroschewski, M. Künzler | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Abstract | This 1st year Laboratory course introduces the students to the basic experimental techniques used in the classical and modern molecular biosciences. In the first year (Praktikum GL BioI) the students will be introduced into the basic concepts and methods of Microbiology, Biochemistry and Molecular Biology in 12 consecutive course days. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Learning objective | Introduction to the biology of unicellular organisms and practical experience with biochemical and molecular biology techniques. General Praktikum-information and course material can be obtained from Moodle. The general Praktikum information (Assignment list and Instructions) will also be sent to the students directly (E-mail). | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Content | This 1st year Laboratory course introduces the students to the entire range of classical and modern molecular biosciences. Students will participate in 12 consecutive course days. The practical will operate in two shifts on Thursday and Friday and requires in addition theoretical preparation via Moodle. morning shift 8:00-13:00 afternoon shift 13:30-18:30 Program Day 1: Handling and detection of microorganisms Day 2: Morphological, biochemical and genetic differentiation of microorganisms Day 3: Biotic interactions and genetics of bacteria Day 4: Metabolism and physiology of bacteria Day 5: DNA extraction, tranformation (E.coli, yeast) Day 6: RNA extraction, Life cycle of eukaryotic cells Day 7: Purification of TAQ polymerase from E coli by ionexchange chromatography Day 8: Characterizing fractions of the purification of TAQ polymerase (SDS-PAGE, WB) Day 9: mRNA splicing in yeast by RT-PCR and purified TAQ polymerase Day 10: Affinity chromatography, protein crystallization Day 11: Enzyme kinetics Day 12: Protein folding, stability and structure | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lecture notes | Laboratory manuals can be downloaded from Moodle | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Prerequisites / Notice | PLEASE NOTE THE FOLLOWING RULES Your attendance is obligatory and you have to attend all 12 Praktikum days of GL BioI. Absences are only acceptable if you are able to provide a Doctor’s certificate. The original Dr's certificate has to be given to Dr. M. Gstaiger (HPM F43) within five days of the absence of the Praktikum day. If there will be any exceptional or important situations then you should directly contact the Director of Studies of D-Biol, who will decide if you are allowed to miss a Praktikum day or not. HIGHLY IMPORTANT!! 1. Due to the increased number of students, the official Praktikum registration has to be done, using myStudies, preferably at the end of HS22 but not later than January 28, 2023. 2. Later registration is NOT possible and can NOT be accepted! 3. The course registration for FS2023 is usually possible at the end of fall semester 2022 and you will obtain an E-mail from the Rectorate when the course registration using myStudies is possible. The 12 course days of the Praktikum Grundlagen Biologie-I will take place Thursday or Friday during the spring semester 2023. Therefore, you have to make sure already now that you will not have any other activities / commitments during these days. The exact course schedule will be communicated before the beginning of the spring semester. PRAKTIKUM DAYS FS23 (Thursdays): 23.02.; 02.03.; 09.03.; 16.03.; 23.03.; 30.03.; 20.04.; 27.04.; 04.05.; 11.05.; 25.05.; 01.06. PRAKTIKUM DAYS FS23 (Fridays): 24.02.; 03.03.; 10.03.; 17.03.; 24.03.; 31.03.; 21.04.; 28.04.; 05.05.; 12.05.; 26.05.; 02.06. No Praktikum during the Easter vacation: 03.04-14.04. |