Elisa Dultz: Catalogue data in Spring Semester 2022

Name Dr. Elisa Dultz
Address
Institut für Biochemie
ETH Zürich, HPM E 14.2
Otto-Stern-Weg 3
8093 Zürich
SWITZERLAND
Telephone+41 44 633 63 70
E-mailelisa.dultz@bc.biol.ethz.ch
DepartmentBiology
RelationshipLecturer

NumberTitleECTSHoursLecturers
551-0128-00LFundamentals of Biology I Restricted registration - show details
Registrations via myStudies until 28.1.2022 at the latest. Subsequent registrations will not be considered.
8 credits8PM. Gstaiger, A. Cléry, E. Dultz, C. H. Giese, R. Kroschewski, M. Künzler
AbstractThis 1st year Laboratory course introduces the students to the basic experimental techniques used in the classical and modern molecular biosciences. In the first year (Praktikum GL BioI) the students will be introduced into the basic concepts and methods of Microbiology, Biochemistry and Molecular Biology in 12 consecutive course days.
Learning objectiveIntroduction to the biology of unicellular organisms and practical experience with biochemical and molecular biology techniques.

General Praktikum-information and course material can be obtained from Moodle

The general Praktikum information (Assignment list, Instructions and Schedule & Performance Sheet) will also be sent to the students directly (E-mail).
ContentThis 1st year Laboratory course introduces the students to the entire range of classical and modern molecular biosciences. Students will participate in 12 consecutive course days. The practical will operate in two shifts on Thursday and Friday.

morning shift 8:00-13:00

afternoon shift 13:30-18:30

Program

Day 1: Handling and detection of microorganisms
Day 2: Morphological, biochemical and genetic differentiation of microorganisms
Day 3: Biotic interactions and genetics of bacteria
Day 4: Metabolism and physiology of bacteria
Day 5: DNA extraction, tranformation (E.coli, yeast)
Day 6: RNA extraction, Life cycle of eukaryotic cells
Day 7: Purification of TAQ polymerase from E coli by ionexchange chromatography
Day 8: Characterizing fractions of the purification of TAQ polymerase (SDS-PAGE, WB)
Day 9: mRNA splicing in yeast by RT-PCR and purified TAQ polymerase
Day 10: Affinity chromatography, protein crystallization and structure
Day 11: Protein folding, protein stability and Enzyme kinetics
Day 12: Protein folding, protein stability and Enzyme kinetics
Lecture notesLaboratory manuals can be downloaded from Moodle
Prerequisites / NoticePLEASE NOTE THE FOLLOWING RULES

Your attendance is obligatory and you have to attend all 12 Praktikum days of GL BioI. Absences are only acceptable if you are able to provide a Doctor’s certificate. The original Dr's certificate has to be given to Dr. M. Gstaiger (HPM F43) within five days of the absence of the Praktikum day.

If there will be any exceptional or important situations then you should directly contact the Director of Studies of D-Biol, who will decide if you are allowed to miss a Praktikum day or not.

HIGHLY IMPORTANT!!

1. Due to the increased number of students, the official Praktikum registration has to be done, using myStudies, preferably at the end of HS21 but not later than January 28, 2022.

2. Later registration is NOT possible and can NOT be accepted!

3. The course registration for FS2022 is usually possible at the end of fall semester 2021 and you will obtain an E-mail from the Rectorate when the course registration using myStudies is possible.

The 12 course days of the Praktikum Grundlagen Biologie-I will take place Thursday or Friday during the spring semester 2022. Therefore, you have to make sure already now that you will not have any other activities / commitments during these days. The exact course schedule will be communicated before the beginning of the spring semester.

PRAKTIKUM DAYS FS22 (Thursdays):

24.02.; 03.03.; 10.03.; 17.03.; 24.03.; 31.03.; 07.04.; 28.04.; 5.05.; 12.05.; 19.05.; 02.06.

PRAKTIKUM DAYS FS22 (Fridays):

25.02.; 04.03.; 11.03.; 18.03.; 25.03.; 1.04.; 8.04.; 29.04.; 06.05.; 13.05.; 20.05.; 03.06.

No Praktikum during the Easter vacation: 11.04-22.04.
551-0339-00LMolecular Mechanisms of Cell Dynamics Restricted registration - show details
Number of participants limited to 18.

The enrolment is done by the D-BIOL study administration.
6 credits7PE. Dultz, Y. Barral, U. Kutay, M. Peter, K. Weis
AbstractApplication of current experimental strategies to study the dynamics of complex and highly regulated cellular processes.
Learning objectiveIn this course, students will
- learn what principles govern cellular dynamics and how these are regulated.
- learn to evaluate and to apply current strategies to study the dynamics of complex and highly regulated cellular processes
ContentDuring this Block-Course, the students will learn to
(1) describe the important mechanisms and regulators of dynamic processes in cells,
(2) perform experimental techniques to quantify dynamic cellular processes,
(3) evaluate and compare experimental strategies and model systems,
(4) formulate and present scientific concepts in an oral presentation.

Topics discussed will include
- mobility in the cell (passive and active)
- compartmentalization (by membranes and via phase separation)
- examples of cell biological processes dependent on mobility and compartmentalization.

Students will work in small groups in individual labs on one research project (8 full days of practical work; every group of students will stay in the same lab during the entire course). The projects are close to the actual research carried out in the participating research groups, but with a clear connection to the subject of the course.
LiteratureDocumentation and recommended literature (review articles and selected primary literature) will be provided during the course.
Prerequisites / NoticeThis course will be taught in english.