Karsten Weis: Katalogdaten im Herbstsemester 2023 |
| Name | Herr Prof. Dr. Karsten Weis |
| Lehrgebiet | Zelluläre Dynamik |
| Adresse | Institut für Biochemie ETH Zürich, HPM E 6 Otto-Stern-Weg 3 8093 Zürich SWITZERLAND |
| Telefon | +41 44 632 30 08 |
| karsten.weis@bc.biol.ethz.ch | |
| Departement | Biologie |
| Beziehung | Ordentlicher Professor |
| Nummer | Titel | ECTS | Umfang | Dozierende | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 551-0337-00L | Cell Biology of the Nucleus  Number of participants limited to 18. The enrolment is done by the D-BIOL study administration. | 6 KP | 7P | R. Kroschewski, Y. Barral, M. Jagannathan, S. Jessberger, K. Weis | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Introduction to the organizational principles of the nucleus using budding yeast, drosophila and vertebrate cells as model systems. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lernziel | The aim of our course is to introduce the students to the organizational principles of the nucleus using budding yeast, drosophila and vertebrate cells as model systems. Emphasis is given to: • Establishment of nuclear identity and nuclear-cytoplasmic communication • Reorganization of the nucleus in aging • Animal cells during the generation of cell diversity and neuronal differentiation By the end of the course, based on lectures, literature reading and practical lab work, the students will be able to formulate open questions concerning the function of the nucleus. Thus, the students will know about the mechanisms and consequences of nuclear-cytoplasmic compartmentalization, DNA clustering in the nucleus and cytoplasm during cell divisions and aging. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Inhalt | During this block-course, the students will - learn how organelles establish and maintain identity with a focus on the nucleus - discover the evolutionary and functional plasticity of the nucleus - design, apply, evaluate and compare experimental strategies Students - in groups of 2 or max. 3 - will be integrated into a research project connected to the subject of the course, within one of the participating research groups. Lectures and technical notes will be given and informal discussions held to provide you with the theoretical background. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Skript | There will be optional papers to be read before the course start. They serve as framework orientation for the practical parts of this block course and will be made accessible to you shortly before the course starts on the relevant Moodle site. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literatur | Documentation and recommended literature (review articles) will be provided during the course. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Kompetenzen |
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| 551-0357-00L | Cellular Matters: Properties, Functions and Applications of Biomolecular Condensates The number of participants is limited to 30 and will only take place with a minimum of 6 participants. The first lecture will serve to form groups of students and assign papers. | 4 KP | 2S | T. Michaels, F. Allain, P. Arosio, Y. Barral, D. Hilvert, M. Jagannathan, R. Mezzenga, G. Neurohr, R. Riek, A. E. Smith, K. Weis, H. Wennemers, weitere Dozierende | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | This Master level course delves into the emerging field of biomolecular condensates - membrane-less organelles in cells. Using interdisciplinary concepts from biology, chemistry, biophysics, and soft matter, we will explore the biological properties of these condensates, their functions in health and disease, and their potentiol as new biomimetic materials for various applications. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lernziel | In the last decade, a novel type of cell compartments called biomolecular condensates have been discovered. This discovery is radically changing our understanding of the cell, its organization, and dynamics. The emerging picture is that the cytoplasm and nucleoplasm are highly complex fluids that can (meta)stably segregate into membrane-less compartments, similary to emulsions. This interdisciplinary course encompasses milestone works and cutting-edge research questions in the young field of biomolecular condensates, including their properties, functions, and applications. The course begins with a lecture series that introduces the topic of condensates to an interdisciplinary audience and provides a theoretical foundation for understanding current research questions in the field. the lecturesprovide a base for student presentations of recent publications in the field, and for research seminars given by course lecturers, who are all active researchers with diverse expertise. Through this exciting interdisciplinary understanding of biomolecular condensates, bridging biology, chemistry, biophysics, and soft matter. Students will not only learn how to critically read and evaluate scientific literature but will also gain valuable experience in giving scientific presentations to an interdisciplinary audience. Each presentation will require an introduction, critical analysis of the results, and a discussion of their significance, allowing student to substantiate their statements with a critical mindset that considers the pros and cons of chosen approaches and methods, as well as any limitations or possible follow-up experiments. This process will enable student to ask relevant querions and actively participate in class discussions, further enhancing their scientific skills. In preparing the presentations, the students will have the unique opportunity to interact closely with each other and with the lecturers, who are all internationally well-established experts, and receive guidance in selectin a topic for the final presentaton and supporting literature. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Inhalt | The topic of biomolecular condensates goes beyond the boundaries of traditional disciplines and requires a multi-disciplinary approach that leverages and cross-fertilizes biology, physical chemistry, biophysics, and soft matter. This course will explore the properties, functions and potentioal applicatons of biomolecular condensates, including their role in neurodegenerative diseases such as Alzheimer's and Parkinson's, as well as their use as smart biomimetic materials. This course is divided into two parts. The fist part will introduce the basic concepts essentialto the study of biomolecular condensates and phase separation. Topics include: fundamental units and scales in soft matter, phase transitions in biology, biopolymers and molecular self-assembly, introduction to active matter. This will establish a foundation for the second part, which will explore milestone works and current research in the field of biomolecular condensates. Each lecture of this second part will consist of: 1) a short literature seminar, where student groups will present and discuss a milestone paper assigned in advance and 2) a research seminar, where one of the course lecturers will present their own state-of-the art research in the field, building upon the milestone literature. At the beginning of the course, student groups will be formed and assigned the milestone papers. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Skript | Lecture slides and some scripts will be provided. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literatur | No compulsory textbooks. Literature will be provided during the course | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kompetenzen |
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| 551-1005-00L | Bioanalytics | 4 KP | 4G | P. Picotti, F. Allain, V. Korkhov, M. Pilhofer, R. Schlapbach, K. Weis, K. Wüthrich, weitere Dozierende | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Der Kurs gibt eine Einführung in die wichtigsten der modernen biologischen Forschung zugrunde liegenden Labortechniken. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lernziel | Für jede der besprochenen Techniken können die Studierenden am Ende des Kurses erklären: a) die physikalischen, chemischen und biologischen Prinzipien die der Technik zugrunde liegen, b) welche Anforderungen Technik an die verwendeten Proben stellt c) welche Rohdaten erhoben werden d) welche Annahmen und Zusatzdaten bei der Interpretation der Daten verwendet werden. e) wie die Daten und die Resultate der Analyse benutzt werden um biologische Fragen zu beantworten. Am Ende des Kurses werden die Studierenden in der Lage sein unter den besprochenen Techniken diejenigen auszuwählen, die am besten geeignet sind um eine bestimmte biologische Frage zu beantworten. Sie werden zusätzlich in der Lage sein die Vor- und Nachteile der in Frage kommenden Techniken zu diskutieren und zu beschreiben wie verschiedene Techniken kombiniert werden können um ein umfassenderes Verständnis der biologische Frage zu erhalten. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Inhalt | Der Kurs wird aus einer Kombination von Vorlesungen, Selbststudiumseinheiten und Übungen bestehen. Der Fokus des Kurses liegt auf folgenden Techniken: -DNA Sequenzierung - Chromatographische Techniken - Massenspektrometrie - UV/Vis- und Fluoreszensspektroskopie - Lichtmikroskopie - Elektronenmikroskopie - Röntgenkristallographie - NMR Spektroskopie | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Skript | Der Kurs wird durch eine Moodle Seite unterstützt auf der für den Kurs notwendigen Materialen zur Verfügung gestellt werden. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kompetenzen |
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