Karsten Weis: Catalogue data in Spring Semester 2021
|Name||Prof. Dr. Karsten Weis|
Institut für Biochemie
ETH Zürich, HPM E 6
|Telephone||+41 44 632 30 08|
|551-0126-00L||Fundamentals of Biology II: Cells||6 credits||5G||K. Weis, F. Allain, Y. Barral, W.‑D. Hardt, U. Kutay, M. Peter, I. Zemp|
|Abstract||The lecture provides an introduction to the function and regulations of cells.|
|Objective||Introduction to the function and regulation of cells|
|Content||The lecture introduces a basic understanding of the structure, organization, function and regulation of the cell. The lecture is divided into two main sections:|
Part 1: Cell Biology of Prokaryotes, evolution, populations
This section covers the general principles of the structure and regulation of prokaryotic cells, and explains the genetics and the evolution of bacteria.
Part II: Unifying concepts in Eukarya
This part of the lecture gives a broad introduction into the general structure of eukaryotic cells and illustrates key concepts such as intracellular architecture, transport mechanisms and the regulation of gene expression in eukaryotes.
|Lecture notes||The newly conceived lecture is supported by scripts.|
|Literature||The lecture is supported by scripts. Furthermore, the textbook "Molecular Biology of the Cell", Alberts et al. 6th edition, Taylor and Francis, and "Brock Biology of Microorganisms", Madigan et al. 15th edition, Pearson can be used as support for the lecture.|
|551-0339-00L||Molecular Mechanisms of Cell Dynamics |
Number of participants limited to 18.
The enrolment is done by the D-BIOL study administration.
General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
|6 credits||7P||E. Dultz, Y. Barral, U. Kutay, M. Peter, K. Weis|
|Abstract||Application of current experimental strategies to study the dynamics of complex and highly regulated cellular processes.|
|Objective||In this course, students will |
- learn what principles govern cellular dynamics and how these are regulated.
- learn to evaluate and to apply current strategies to study the dynamics of complex and highly regulated cellular processes
|Content||During this Block-Course, the students will learn to|
(1) describe the important mechanisms and regulators of dynamic processes in cells,
(2) perform experimental techniques to quantify dynamic cellular processes,
(3) evaluate and compare experimental strategies and model systems,
(4) formulate and present scientific concepts in an oral presentation.
Topics discussed will include
- mobility in the cell (passive and active)
- compartmentalization (by membranes and via phase separation)
- examples of cell biological processes dependent on mobility and compartmentalization.
Students will work in small groups in individual labs on one research project (8 full days of practical work; every group of students will stay in the same lab during the entire course). The projects are close to the actual research carried out in the participating research groups, but with a clear connection to the subject of the course.
|Literature||Documentation and recommended literature (review articles and selected primary literature) will be provided during the course.|
|Prerequisites / Notice||This course will be taught in english.|
|551-1298-00L||Genetics, Genomics, Bioinformatics||4 credits||2V + 2U||E. Hafen, C. Beyer, B. Christen, U. K. Genick, J. Piel, R. Schlapbach, G. Schwank, S. Sunagawa, K. Weis, A. Wutz|
|Abstract||The course provides the basis of modern genetics, genomics and bioinformatics. A special focus is placed on the use of these tools for the understanding of biological processes in bacteria, model organisms and humans. The unit uses the principle of blended learning consisting of self-study modules in Moodle, tasks and input lectures by experts from the department.|
|Objective||At the end of this course you know the most important genetic tools in different organisms. You can use the essential methods in bioinformatics by using online tools. You know the advantages and disadvantages of various model organisms to understand biological processes. You know the various mutagenesis methods and other tools to disrupt gene function and can discuss their merits and drawbacks. You are aware of the difficulties in choosing a phenotype for selection in a mutagenesis experiment. Finally, you can describe how you would study a specific biological process by choosing a model organism and the appropriate genetic or genomic tools.|
|Content||The appearance and function of an organism (phenotype) is determined by the interplay between its genome (genotype) and the environment: Genotype + environment = phenotype. Understanding these interactions to the point where we can ultimately predict the phenotype from knowledge of the genotype and environmental factors is one oft the great challenges of biology.|
In the course Bio IA you learnt about the composition and function of the genome and how it is inherited. The goal of this course is that you learn how genetic, genomic and bioinformatics methods are used to understand biological processes (the connection between genotype and phenotype).
You will start by refreshing and deepening your knowledge of the basic principles of genetics and genomics in an interactive learning modules on the Moodle platform. This is followed by an introduction of the basic tools of bioinformatics and genomic analysis.
After you have mastered the basic principles you will learn how to study biological processes either by inactivating specific genes or by randomly mutagenizing the entire genome. You will be introduced to different model organisms (bacteria, yeast, Drosophila) and humans.
At the end of this first part of the course, you will test your knowledge by working with a group of fellow students to design your own genetic study.
Conventional genetic methods rely on the alteration of the function of single genes and on the observation of the effect on the organism (phenotype). Based on the observed phenotype one deduces the normal function of the gene. However, this is a strong simplification. Even if environmental factors are controlled, phenotypes are very rarely controlled by a single gene. It is therefore important to understand the influence of the entire genome in conjunction with environmental factors on a given phenotype (e.g. human disease). Modern methods in genomics now permit first approaches in this direction. Therefore, the focus of the second part of the unit is on genomics methods. You learn, how the influence of the entire genome on a specific phenotype is detected and what challenges are involved in the analysis and the interpretation of the results. We will examine these methods in model organisms and humans. You will also learn how the genome of cancer cells changes under the constant selection for these cells to survive and how this genome analysis provides new insights into diagnosis and therapy.
This course is based on active learning. Each week consists of a learning unit with clearly defined learning goals. In the first two hours you will learn the basics from texts, videos and questionnaires on the Moodle platform. In the third lecture an expert on the topic of the week (e.g. genetic screens in yeast) from the department will give an input lecture that builds on the basic knowledge that you acquired. In the fourth lecture you will discuss the tests and topics of the week with the expert. During the semester you will have access to assistants and lecturers via the Moodle online forum.
|Lecture notes||The learning material and slides of the input lectures are available on Moodle. There you will also find further information (articles, links, videos).|
|Literature||All texts and references will be available on Moodle. To follow the most recent developments in this rapidly evolving field follow the following experts on Twitter:|
|Prerequisites / Notice||The course builds on the course Bio IA, in particular on that course's content regarding genetics and genomics. The course is based on self-learning units on Moodle, input lectures by experts from D-BIOL and exercises.|