Martin Pilhofer: Catalogue data in Autumn Semester 2023 |
| Name | Prof. Dr. Martin Pilhofer |
| Field | Cryo-Electron Microscopy |
| Address | Inst. f. Molekularbiol.u.Biophysik ETH Zürich, HPK F 13 Otto-Stern-Weg 5 8093 Zürich SWITZERLAND |
| Telephone | +41 44 633 39 63 |
| pilhofer@biol.ethz.ch | |
| Department | Biology |
| Relationship | Full Professor |
| Number | Title | ECTS | Hours | Lecturers | ||||||||||||||||||||||||||||||||
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| 551-0313-00L | Microbiology (Part I) | 3 credits | 2V | W.‑D. Hardt, L. Eberl, B. Nguyen, J. Piel, M. Pilhofer, A. Vagstad | ||||||||||||||||||||||||||||||||
| Abstract | Advanced lecture class providing a broad overview on bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis. | |||||||||||||||||||||||||||||||||||
| Learning objective | This concept class will be based on common concepts and introduce to the enormous diversity among bacteria and archaea. It will cover the current research on bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis. | |||||||||||||||||||||||||||||||||||
| Content | Advanced class covering the state of the research in bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis. | |||||||||||||||||||||||||||||||||||
| Lecture notes | Updated handouts will be provided during the class. | |||||||||||||||||||||||||||||||||||
| Literature | Current literature references will be provided during the lectures. | |||||||||||||||||||||||||||||||||||
| Prerequisites / Notice | English The lecture "Grundlagen der Biologie II: Mikrobiologie" is the basis for this advanced lecture. | |||||||||||||||||||||||||||||||||||
| 551-1005-00L | Bioanalytics  | 4 credits | 4G | P. Picotti, F. Allain, V. Korkhov, M. Pilhofer, R. Schlapbach, K. Weis, K. Wüthrich, further lecturers | ||||||||||||||||||||||||||||||||
| Abstract | The course will introduce students to a selected set of laboratory techniques that are foundational to modern biological research. | |||||||||||||||||||||||||||||||||||
| Learning objective | For each of the techniques covered in the course, the students will be able to explain: a) the physical, chemical and biological principles underlying the technique, b) the requirements for the sample, c) the type of raw data collected by the technique, d) the assumptions and auxiliarry information used in the interpretation of the data and e) how these data can be used to answer a given biological question. By the end of the course the students will be able to select the appropriate experimental technique to answer a given biological problem and will be able to discuss the advantages and limitations of individual techniques as well as how different techniques can be combined to gain a more complete understanding of a given biological questions. | |||||||||||||||||||||||||||||||||||
| Content | The course will be based on a combination of lectures, selfstudy elements and exercises. The focus will be on the following experimental techniques: - DNA sequencing - chromatography - mass-spectrometry - UV/Vis and fluorescence spectrometry - light microscopy - electron microscopy - X-ray crystallography - NMR spectroscopy | |||||||||||||||||||||||||||||||||||
| Lecture notes | The course is supported by a Moodle page that gives access to all supporting materials necessary for the course. | |||||||||||||||||||||||||||||||||||
| Competencies |
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| 551-1403-00L | Imaging Bacterial Cells in a Native State by Electron Cryotomography  Number of participants limited to 15. The enrolment is done by the D-BIOL study administration. | 6 credits | 7P | M. Pilhofer, G. Weiss | ||||||||||||||||||||||||||||||||
| Abstract | The goal is to acquire the techniques to image bacteria by electron cryotomography, resolving their structure in a native state, in 3D, and to macromolecular resolution. In a small group, students will perform wet lab experiments, data collection with stat-of-the-art equipment, data processing and analyses. The key method and its application in bacterial cell biology will be introduced by lectures | |||||||||||||||||||||||||||||||||||
| Learning objective | Students will acquire the skills to cultivate bacteria, plunge-freeze samples for cryotomography, collect data using an electron cryomicroscope, process raw data, analyze tomograms, perform subtomogram averaging, model structures of interest, and generate movies for visualization. https://www.mol.biol.ethz.ch/groups/pilhofer_group/ | |||||||||||||||||||||||||||||||||||
| 752-4001-00L | Microbiology Some parts of the lecture will be taught in English. | 2 credits | 2V | M. Schuppler, M. La Fortezza, M. Pilhofer, S. Robinson | ||||||||||||||||||||||||||||||||
| Abstract | Teaching of basic knowledge in microbiology with main focus on Microbial Cell Structure and Function, Molecular Genetics, Microbial Growth, Metabolic Diversity, Phylogeny and Taxonomy, Prokaryotic Diversity, Human-Microbe Interactions, Biotechnology. | |||||||||||||||||||||||||||||||||||
| Learning objective | Teaching of basic knowledge in microbiology. | |||||||||||||||||||||||||||||||||||
| Content | Der Schwerpunkt liegt auf den Themen: Bakterielle Zellbiologie, Molekulare Genetik, Wachstumsphysiologie, Biochemische Diversität, Phylogenie und Taxonomie, Prokaryotische Vielfalt, Interaktion zwischen Menschen und Mikroorganismen sowie Biotechnologie. | |||||||||||||||||||||||||||||||||||
| Lecture notes | Wird von den jeweiligen Dozenten ausgegeben. | |||||||||||||||||||||||||||||||||||
| Literature | Die Behandlung der Themen erfolgt auf der Basis des Lehrbuchs Brock, Biology of Microorganisms | |||||||||||||||||||||||||||||||||||
| Competencies |
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