Martin Pilhofer: Catalogue data in Autumn Semester 2022

Name Prof. Dr. Martin Pilhofer
FieldCryo-Electron Microscopy
Inst. f. Molekularbiol.u.Biophysik
ETH Zürich, HPK F 13
Otto-Stern-Weg 5
8093 Zürich
Telephone+41 44 633 39 63
RelationshipFull Professor

551-0313-00LMicrobiology (Part I)3 credits2VW.‑D. Hardt, L. Eberl, B. Nguyen, J. Piel, M. Pilhofer, A. Vagstad
AbstractAdvanced lecture class providing a broad overview on bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis.
ObjectiveThis concept class will be based on common concepts and introduce to the enormous diversity among bacteria and archaea. It will cover the current research on bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis.
ContentAdvanced class covering the state of the research in bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis.
Lecture notesUpdated handouts will be provided during the class.
LiteratureCurrent literature references will be provided during the lectures.
Prerequisites / NoticeEnglish
The lecture "Grundlagen der Biologie II: Mikrobiologie" is the basis for this advanced lecture.
551-1005-00LBioanalytics Information 4 credits4GP. Picotti, F. Allain, V. Korkhov, M. Pilhofer, R. Schlapbach, K. Weis, K. Wüthrich, further lecturers
AbstractThe course will introduce students to a selected set of laboratory techniques that are foundational to modern biological research.
ObjectiveFor each of the techniques covered in the course, the students will be able to explain:
a) the physical, chemical and biological principles underlying the technique,
b) the requirements for the sample,
c) the type of raw data collected by the technique,
d) the assumptions and auxiliarry information used in the interpretation of the data and
e) how these data can be used to answer a given biological question.
By the end of the course the students will be able to select the appropriate experimental technique to answer a given biological problem and will be able to discuss the
advantages and limitations of individual techniques as well as how different techniques can be combined to gain a more complete understanding of a given biological questions.
ContentThe course will be based on a combination of lectures, selfstudy elements and exercises.

The focus will be on the following experimental techniques:

- DNA sequencing
- chromatography
- mass-spectrometry
- UV/Vis and fluorescence spectrometry
- light microscopy
- electron microscopy
- X-ray crystallography
- NMR spectroscopy
Lecture notesThe course is supported by a Moodle page that gives access to all supporting materials necessary for the course.
Subject-specific CompetenciesConcepts and Theoriesassessed
Techniques and Technologiesassessed
Method-specific CompetenciesAnalytical Competenciesassessed
Media and Digital Technologiesfostered
Project Managementfostered
Personal CompetenciesCreative Thinkingassessed
Critical Thinkingassessed
551-1403-00LImaging Bacterial Cells in a Native State by Electron Cryotomography Restricted registration - show details
Number of participants limited to 15.
The enrolment is done by the D-BIOL study administration.
6 credits7PM. Pilhofer, G. Weiss
AbstractThe goal is to acquire the techniques to image bacteria by electron cryotomography, resolving their structure in a native state, in 3D, and to macromolecular resolution. In a small group, students will perform wet lab experiments, data collection with stat-of-the-art equipment, data processing and analyses. The key method and its application in bacterial cell biology will be introduced by lectures
ObjectiveStudents will acquire the skills to cultivate bacteria, plunge-freeze samples for cryotomography, collect data using an electron cryomicroscope, process raw data, analyze tomograms, perform subtomogram averaging, model structures of interest, and generate movies for visualization.
551-1619-00LStructural Biology
Does not take place this semester.
1 credit1KR. Glockshuber, F. Allain, N. Ban, K. Locher, M. Pilhofer, E. Weber-Ban, K. Wüthrich
AbstractThe course consists of a series of research seminars on Structural Biology, Biochemistry and Biophysics, given by both scientists of the National Center of Competence in Research (NCCR) in Structural Biology and external speakers. Information on the individual seminars is provided on the following websites:
ObjectiveThe goal of this course is to provide doctoral and postdoctoral students with a broad overview on the most recent developments in biochemistry, structural biology and biophysics.