| Nummer | Titel | ECTS | Umfang | Dozierende |
|---|
| 551-1402-00L | Biophysics and Macromolecular Mechanisms | 4 KP | 2V | R. Glockshuber,
T. Ishikawa,
B. Schuler,
D. Veprintsev,
V. Vogel,
E. Weber-Ban |
| Kurzbeschreibung | The course is focussed on biophysical methods for characterising conformational transitions and reaction mechanisms of proteins and biological mecromolecules, with focus on methods that have not been covered in the Biology Bachelor Curriculum. |
| Lernziel | The goal of the course is to give the students a broad overview on biopyhsical techniques available for studying conformational transitions and complex reaction mechanisms of biological macromolecules. The course is particularly suited for students enrolled in the Majors "Structural Biology and Biophysics", "Biochemistry" and "Chemical Biology" of the Biology MSc curriculum, as well as for MSc students of Chemistry and Interdisciplinary Natural Sciences". |
| Inhalt | The biophysical methods covered in the course include advanced reaction kinetics, methods for the thermodynamic and kinetic analysis of protein-ligand interactions, classical and dynamic light scattering, analytical ultracentrifugation, spectroscopic techniques such as fluorescence anisotropy, fluorescence resonance energy transfer (FRET) and single molecule fluorescence spectrosopy, modern electron microscopy techniques, atomic force microscopy, and isothermal and differential scanning calorimetry. |
| Skript | Course material from the individual lecturers wil be made available at the sharepoint website
https://team.biol.ethz.ch/e-learn/551-1402-00L |
| Voraussetzungen / Besonderes | Finished BSc curriculum in Biology, Chemistry or Interdisciplinary Natural Sciences. The course is also adequate for doctoral students with research projects in structural biology, biophysics, biochemistry and chemical biology. |
| 551-1556-00L | X-Ray Crystallographic Structure Determination and Biophysics   | 6 KP | 7G | K. Locher,
G. Schertler,
D. Veprintsev |
| Kurzbeschreibung | This course will familiarize the students with techniques used for the biophysical and structural characterization of proteins. The students will carry out biophysical characterization of the proteins with dynamic light scattering and CD spectroscopy. |
| Lernziel | The course aims at introducing the principles of protein X-ray crystallography as well as other techniques used in the biophysical characterization of proteins. Students will get an opportunity to conduct hands-on experiments and also use computational techniques. |
| Inhalt | Micro-calorimetry and fluorescence anisotropy measurements will be used to study protein peptide interactions. The course also includes a demonstration of the Synchrotron capabilities at the Paul Scherrer Institute (SLS). Students will crystallize a protein, collect X-ray diffraction data using an in-house X-ray source, and build an atomic model into the electron density map. |
| Voraussetzungen / Besonderes | The students will be both at ETH Honggerberg and the Paul Scherrer Institute (PSI) at Villigen. Transport will be organized by rental car or by public transportation. |
