Ludovic Gillet: Catalogue data in Autumn Semester 2022

Name Dr. Ludovic Gillet
Address
Inst. f. Molekulare Systembiologie
ETH Zürich, HPM F 42
Otto-Stern-Weg 3
8093 Zürich
SWITZERLAND
Telephone+41 44 633 31 91
Fax+41 44 633 10 51
E-mailgillet@imsb.biol.ethz.ch
DepartmentBiology
RelationshipLecturer

NumberTitleECTSHoursLecturers
551-0352-00LIntroduction to Mass Spectrometry-Based Proteomics Restricted registration - show details
Number of participants limited to 12.

The enrolment is done by the D-BIOL study administration.
6 credits7PL. Gillet, P. Picotti
AbstractProtein Analysis by Mass Spectrometry
The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis.
ObjectiveHow to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up)
Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments)
Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares)
Perform quantitative proteomic analysis (label-free and labeled analyses)
Analyze/interpret the data to find up/down regulated proteins