Ludovic Gillet: Catalogue data in Spring Semester 2022 |
| Name | Dr. Ludovic Gillet |
| Address | Inst. f. Molekulare Systembiologie ETH Zürich, HPM F 42 Otto-Stern-Weg 3 8093 Zürich SWITZERLAND |
| Telephone | +41 44 633 31 91 |
| Fax | +41 44 633 10 51 |
| gillet@imsb.biol.ethz.ch | |
| Department | Biology |
| Relationship | Lecturer |
| Number | Title | ECTS | Hours | Lecturers | |
|---|---|---|---|---|---|
| 551-0224-00L | Advanced Proteomics  For master students from the 2nd semester on, also doctoral candidates and post docs. | 4 credits | 6G | P. Picotti, L. Gillet, A. Leitner, P. Pedrioli | |
| Abstract | Goal of the course is to analyze current and newly emerging technologies and approaches in protein and proteome analysis with regard to their application in biology, biotechnology and medicine. Format: Introduction by instructor followed by discussions stimulated by reading assignments and exercises. | ||||
| Learning objective | To discuss current and newly emerging technologies and approaches in protein and proteome analysis with regard to their applications in biology, biotechnology, medicine and systems biology. | ||||
| Content | Block course teaching current methods for the acquisition and processing of proteomic datasets. | ||||
| Prerequisites / Notice | Number of people: Not exceeding 30. Students from ETHZ, Uni Zurich and University of Basel Non-ETH students must register at ETH Zurich as special students http://www.rektorat.ethz.ch/students/admission/auditors/index_EN | ||||
| 551-0352-00L | Introduction to Mass Spectrometry-Based Proteomics Number of participants limited to 12. The enrolment is done by the D-BIOL study administration. | 6 credits | 7P | L. Gillet, P. Picotti | |
| Abstract | Protein Analysis by Mass Spectrometry The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis. | ||||
| Learning objective | How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up) Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments) Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares) Perform quantitative proteomic analysis (label-free and labeled analyses) Analyze/interpret the data to find up/down regulated proteins | ||||