Gebhard Schertler: Katalogdaten im Frühjahrssemester 2020
|Name||Herr Prof. em. Dr. Gebhard Schertler|
Professur für Strukturbiologie
Paul Scherrer Institut
5232 Villigen PSI
|Telefon||+41 56 310 42 65|
|551-0103-AAL||Fundamentals of Biology II: Cell Biology|
Belegung ist NUR erlaubt für MSc Studierende, die diese Lerneinheit als Auflagenfach verfügt haben.
Alle anderen Studierenden (u.a. auch Mobilitätsstudierende, Doktorierende) können diese Lerneinheit NICHT belegen.
|5 KP||11R||U. Kutay, Y. Barral, E. Hafen, G. Schertler, U. Suter, S. Werner|
|Kurzbeschreibung||The goal of this course is to provide students with a wide general understanding in cell biology. With this material as a foundation, students have enough of a cell biological basis to begin their specialization not only in cell biology but also in related fields such as biochemistry, microbiology, pharmacological sciences, molecular biology, and others.|
|Lernziel||The goal of this course is to provide students with a wide general understanding cell biology. With this material as a foundation, students have enough of a cell biological basis to begin their specialization not only in cell biology but also in related fields such as biochemistry, microbiology, pharmacological sciences, molecular biology, and others.|
|Inhalt||The focus is animal cells and the development of multicellular organisms with a clear emphasis on the molecular basis of cellular structures and phenomena. The topics include biological membranes, the cytoskeleton, protein sorting, energy metabolism, cell cycle and division, viruses, extracellular matrix, cell signaling, embryonic development and cancer research.|
|Literatur||Alberts et al. 'Molecular Biology of the Cell' 6th edition, 2014, ISBN 9780815344322 (hard cover) and ISBN 9780815345244 (paperback).|
Analyzing cells & molecules / Gebhard Schertler/8/ 439-463;
Membrane structure / Gebhard Schertler/ 10/ 565-595;
Compartments and Sorting/ Ulrike Kutay/12+14+6/641-694/755-758/782-783/315-320/325 -333/Table 6-2/Figure6-20, 6-21, 6-32, 6-34;
Intracellular Membrane Traffic/ Ulrike Kutay/13/695-752;
The Cytoskeleton/ Ulrike Kutay/ 16/889 - 948 (only the essentials);
Membrane Transport of Small Molecules and the Electrical Properties of Membranes /Sabine Werner/11/597 - 633;
Mechanisms of Cell Communication / Sabine Werner/15/813-876;
Cancer/ Sabine Werner/20/1091-1141;
Cell Junctions and Extracellular Matrix/Ueli Suter / 1035-1081;
Stem Cells and Tissue Renewal/Ueli Suter /1217-1262;
Development of Multicellular organisms/ Ernst Hafen/ 21/ 1145-1179 /1184-1198/1198-1213;
Cell Migration/Joao Matos/951-960;
Cell Death/Joao Matos/1021-1032;
Cell Cycle/chromosome segregation/Cell division/Meiosis/Joao Matos/ 963-1018.
|Voraussetzungen / Besonderes||none|
|551-1554-00L||Multigene Expression in Mammalian Cells |
Number of participants limited to 5.
The enrolment is done by the D-BIOL study administration.
|6 KP||7G||P. Berger, G. Schertler|
|Kurzbeschreibung||Genetic engineering of mammalian cells with multiple expression cassettes is an essential need in contemporary cell biology. It is useful for protein expression for structural studies, the reprogramming of somatic cells, or for the expression of several fluorescently-tagged sensors. In this course, we use MultiLabel (Kriz et al., Nat. Commun., 2010) to create multigene expression plasmids.|
|Lernziel||Students will learn to design and clone multigene expression constructs for mammalian cells. The functionality of the constructs will be tested by immunofluorescence microscopy or Western blotting.|
|Inhalt||We will clone fluorescently-tagged markers for subcellular compartments, assemble them to a multigene expression construct and transfect them into mammalian cells. These markers of subcellular compartments will be used to study the trafficking of activated receptors (e.g. serotonin receptor). Pictures will be taken on our microscopes and then we will quantify colocalization.|
|551-1556-00L||Macromolecular Structure Determination Using Modern Methods |
Number of participants limited to 11 in the 3rd semester quarter of the spring semester
Number of participants limited to 12 in the 4th semester quarter of the spring semester
The block course will only take place with a minimum of 4 participants.
The enrolment is done by the D-BIOL study administration.
|6 KP||7G||K. Locher, G. Schertler|
|Kurzbeschreibung||This course will expose the students to two prominent techniques for high-resolution structural characterization of biological macromolecules. The students will have the opportunity to get hands-on experience in either cryo-electron microscopy (ETH) or X-ray crystallography (PSI).|
|Lernziel||The goal of this course is to introduce the students to the principles of high-resolution structure determination. Students will conduct hands-on experiments and use computational techniques for data processing.|
|Inhalt||At the ETH the students will prepare and vitrify a protein and then image it on a cryo-TEM. Next, the students will process the data and build an atomic model into the EM map. |
At the PSI the students will purify and crystallize a membrane protein, collect X-ray diffraction data using synchrotron X-ray source or with cryo-EM, analyze and build an atomic model into a density map. They will refine this model and interpret and illustrate the determined structure. The course work is trying to present insights in the use of structural information. The course also includes a demonstration of the Synchrotron capabilities at the Paul Scherrer Institute (SLS).
|Voraussetzungen / Besonderes||The students will be split into two groups for the practical part of the work: One group will work at ETH Hönggerberg, the other at the Paul Scherrer Institute (PSI) at Villigen. All students will spend one full day at the PSI for a tour of the facilities, including a visit of the synchrotron beam lines of the Swiss Light Source SLS.|
The students joining the ETH Hönggerberg group will spend the majority of the time on data processing and are therefore expected to have some basic knowledge of bash terminal commands. Basic physics, optics and linear algebra knowledge is also helpful. By the end of the course, the students will be expected to understand concepts such as the difference between Fourier and real space, image formation, contrast transfer, fast Fourier transfer and Fourier shell correlation.