Gebhard Schertler: Catalogue data in Spring Semester 2015 |
| Name | Prof. em. Dr. Gebhard Schertler |
| Field | Structural Biology |
| Address | Professur für Strukturbiologie OSRA/007 Paul Scherrer Institut 5232 Villigen PSI SWITZERLAND |
| Telephone | +41 56 310 42 65 |
| gebhard.schertler@biol.ethz.ch | |
| URL | https://www.psi.ch/bio/schertler-group-research |
| Department | Biology |
| Relationship | Professor emeritus |
| Number | Title | ECTS | Hours | Lecturers | |
|---|---|---|---|---|---|
| 551-0103-AAL | Fundamentals of Biology II: Cell Biology Enrolment only for MSc students who need this course as additional requirement. | 5 credits | 11R | U. Kutay, Y. Barral, E. Hafen, G. Schertler, U. Suter, S. Werner | |
| Abstract | The goal of this course is to provide students with a wide general understanding in cell biology. With this material as a foundation, students have enough of a cell biological basis to begin their specialization not only in cell biology but also in related fields such as biochemistry, microbiology, pharmacological sciences, molecular biology, and others. | ||||
| Learning objective | The goal of this course is to provide students with a wide general understanding cell biology. With this material as a foundation, students have enough of a cell biological basis to begin their specialization not only in cell biology but also in related fields such as biochemistry, microbiology, pharmacological sciences, molecular biology, and others. | ||||
| Content | The focus is animal cells and the development of multicellular organisms with a clear emphasis on the molecular basis of cellular structures and phenomena. The topics include biological membranes, the cytoskeleton, protein sorting, energy metabolism, cell cycle and division, viruses, extracellular matrix, cell signaling, embryonic development and cancer research. | ||||
| Literature | Alberts et al. ‘Molecular Biology of the Cell’ Fifth edition, 2008 ISBN 978-0-8153-4105-5 (hard cover) and ISBN 978-0-8153-4106-2 (paperback). Topic/Lecturer/Chapter/Pages: Introduction to Cell Biology/Gebhard Schertler/1+2+3+4/1-193; Cellular compartments/Gebhard Schertler/12/695-748; Membrane lipids/Gebhard Schertler/10/617-629; Working with cells/Ulrike Kutay/9/579-613; Mitochondria/Ulrike Kutay/12+14/695-703/713-723/815-818/856-860; Chloroplasts, peroxisomes/Ulrike Kutay/12+14/695-703/713-723/840-844/856-860; Structure and dynamics of the nucleus/Ulrike Kutay/6+12/362-366/704-706/710-712; Membrane proteins/Gebhard Schertler/10/629-650; Working with membranes/Gebhard Schertler/9/579-615; Nuclear transport of proteins/Ulrike Kutay/12/706-711; RNA processing and nuclear export/Ulrike Kutay/6/345-353/357-366/369; Endoplasmic reticulum/Ulrike Kutay/12/723-745; Vesicular transport/Ulrike Kutay/13/749-766; From the ER through the Golgi/Ulrike Kutay/13/766-779; From the TGN to Lysosomes and the plasma membrane/Ulrike Kutay/13/779-787/799-809; The plasma membrane and endocytosis/Ulrike Kutay/13/787-799; Introduction to the cytoskeleton/Ulrike Kutay/16/965-1035; Microtubules/Ulrike Kutay/16/965-1035; Actin/Muscle/Ulrike Kutay/16/965-1035; Cell polarization and migration/Yves Barral/16/1036-1052; Introduction to the cell cycle/Yves Barral/17/1053-1070; MPF and the cell cycle control machinery/Yves Barral/17/1053-1070; Mechanisms of chromosome segregation/Yves Barral/17/1070-1090; Cell division/Yves Barral/17/1090-1101; Apoptosis/Yves Barral/18/1115-1127; Membrane transport passive and active/Sabine Werner/11/651-667; Ion channels, action potential/Sabine Werner/11/667-687; General principles of signalling/Sabine Werner/15/879-903; Nuclear receptors, G-protein coupled receptors/Sabine Werner/15/879-921; Cell signalling; G-protein coupled receptors/Sabine Werner/15/904-921; Cell signalling; Receptor tyrosine kinases/Sabine Werner/15/921-938; Cell signalling; Tyrosine kinase associated receptors/Sabine Werner/15/921-938; Cell signalling; Receptor serine threonine kinases/Sabine Werner/15/939-944; Signalling through proteolysis/Sabine Werner/15/946-954; Cancer Biology/Sabine Werner/20/1205-1267; Cell-Cell Interactions/Ueli Suter/19/1131-1195; Extracellular Matrix/Ueli Suter/19/1131-1195; Regeneration / Stem Cells/Ueli Suter/23/1417-1484; Germ Cells and Sex Determination/Ernst Hafen/21/1269-1304; Development/Ernst Hafen/22/1305-1417 | ||||
| Prerequisites / Notice | none | ||||
| 551-1554-00L | Multigene Expression in Mammalian Cells  Number of participants limited to 4. | 6 credits | 7G | P. Berger, G. Schertler | |
| Abstract | Genetic engineering of mammalian cells with multiple expression cassettes is an essential need in contemporary cell biology. It is useful for protein expression for structural studies, the reprogramming of somatic cells, or for the expression of several fluorescently-tagged sensors. In this course, we use MultiLabel (Kriz et al., Nat. Commun., 2010) to create multigene expression plasmids. | ||||
| Learning objective | Students will learn to design and clone multigene expression constructs for mammalian cells. The functionality of the constructs will be tested by immunofluorescence microscopy or Western blotting. | ||||
| Content | We will clone fluorescently-tagged markers for subcellular compartments, assemble them to a multigene expression construct and transfect them into mammalian cells. These markers of subcellular compartments will be used to study the trafficking of activated receptors (e.g. serotonin receptor). Pictures will be taken on our microscopes and then we will quantify colocalization. | ||||
| Lecture notes | none | ||||
| 551-1556-00L | X-Ray Crystallographic Structure Determination and Biophysics  Number of participants limited to 8 per quarter | 6 credits | 7G | K. Locher, G. Schertler, D. Veprintsev | |
| Abstract | This course will familiarize the students with techniques used for the biophysical and structural characterization of proteins. The students will carry out biophysical characterization of the proteins with dynamic light scattering and CD spectroscopy. | ||||
| Learning objective | The course aims at introducing the principles of protein X-ray crystallography as well as other techniques used in the biophysical characterization of proteins. Students will get an opportunity to conduct hands-on experiments and also use computational techniques. | ||||
| Content | Micro-calorimetry and fluorescence anisotropy measurements will be used to study protein peptide interactions. The course also includes a demonstration of the Synchrotron capabilities at the Paul Scherrer Institute (SLS). Students will crystallize a protein, collect X-ray diffraction data using an in-house X-ray source, and build an atomic model into the electron density map. | ||||
| Prerequisites / Notice | The students will be both at ETH Honggerberg and the Paul Scherrer Institute (PSI) at Villigen. Transport will be organized by rental car or by public transportation. | ||||