Gebhard Schertler: Katalogdaten im Frühjahrssemester 2018

NameHerr Prof. em. Dr. Gebhard Schertler
LehrgebietStrukturbiologie
Adresse
Professur für Strukturbiologie
OSRA/007
Paul Scherrer Institut
5232 Villigen PSI
SWITZERLAND
Telefon+41 56 310 42 65
E-Mailgebhard.schertler@biol.ethz.ch
URLhttps://www.psi.ch/bio/schertler-group-research
DepartementBiologie
BeziehungProfessor emeritus

NummerTitelECTSUmfangDozierende
551-0103-AALFundamentals of Biology II: Cell Biology
Belegung ist NUR erlaubt für MSc Studierende, die diese Lerneinheit als Auflagenfach verfügt haben.

Alle anderen Studierenden (u.a. auch Mobilitätsstudierende, Doktorierende) können diese Lerneinheit NICHT belegen.
5 KP11RU. Kutay, Y. Barral, E. Hafen, G. Schertler, U. Suter, S. Werner
KurzbeschreibungThe goal of this course is to provide students with a wide general understanding in cell biology. With this material as a foundation, students have enough of a cell biological basis to begin their specialization not only in cell biology but also in related fields such as biochemistry, microbiology, pharmacological sciences, molecular biology, and others.
LernzielThe goal of this course is to provide students with a wide general understanding cell biology. With this material as a foundation, students have enough of a cell biological basis to begin their specialization not only in cell biology but also in related fields such as biochemistry, microbiology, pharmacological sciences, molecular biology, and others.
InhaltThe focus is animal cells and the development of multicellular organisms with a clear emphasis on the molecular basis of cellular structures and phenomena. The topics include biological membranes, the cytoskeleton, protein sorting, energy metabolism, cell cycle and division, viruses, extracellular matrix, cell signaling, embryonic development and cancer research.
LiteraturAlberts et al. 'Molecular Biology of the Cell' 6th edition, 2014, ISBN 9780815344322 (hard cover) and ISBN 9780815345244 (paperback).

Topic/Lecturer/Chapter/Pages:

Analyzing cells & molecules / Gebhard Schertler/8/ 439-463;
Membrane structure / Gebhard Schertler/ 10/ 565-595;
Compartments and Sorting/ Ulrike Kutay/12+14+6/641-694/755-758/782-783/315-320/325 -333/Table 6-2/Figure6-20, 6-21, 6-32, 6-34;
Intracellular Membrane Traffic/ Ulrike Kutay/13/695-752;
The Cytoskeleton/ Ulrike Kutay/ 16/889 - 948 (only the essentials);
Membrane Transport of Small Molecules and the Electrical Properties of Membranes /Sabine Werner/11/597 - 633;
Mechanisms of Cell Communication / Sabine Werner/15/813-876;
Cancer/ Sabine Werner/20/1091-1141;
Cell Junctions and Extracellular Matrix/Ueli Suter / 1035-1081;
Stem Cells and Tissue Renewal/Ueli Suter /1217-1262;
Development of Multicellular organisms/ Ernst Hafen/ 21/ 1145-1179 /1184-1198/1198-1213;
Cell Migration/Joao Matos/951-960;
Cell Death/Joao Matos/1021-1032;
Cell Cycle/chromosome segregation/Cell division/Meiosis/Joao Matos/ 963-1018.
Voraussetzungen / Besonderesnone
551-1554-00LMultigene Expression in Mammalian Cells Belegung eingeschränkt - Details anzeigen
Number of participants limited to 5.

The enrolment is done by the D-BIOL study administration.
6 KP7GP. Berger, G. Schertler
KurzbeschreibungGenetic engineering of mammalian cells with multiple expression cassettes is an essential need in contemporary cell biology. It is useful for protein expression for structural studies, the reprogramming of somatic cells, or for the expression of several fluorescently-tagged sensors. In this course, we use MultiLabel (Kriz et al., Nat. Commun., 2010) to create multigene expression plasmids.
LernzielStudents will learn to design and clone multigene expression constructs for mammalian cells. The functionality of the constructs will be tested by immunofluorescence microscopy or Western blotting.
InhaltWe will clone fluorescently-tagged markers for subcellular compartments, assemble them to a multigene expression construct and transfect them into mammalian cells. These markers of subcellular compartments will be used to study the trafficking of activated receptors (e.g. serotonin receptor). Pictures will be taken on our microscopes and then we will quantify colocalization.
Skriptnone
551-1556-00LMacromolecular Structure Determination Using Modern Methods Belegung eingeschränkt - Details anzeigen
Number of participants limited to 11 in the 3rd semester quarter of the spring semester

Number of participants limited to 12 in the 4th semester quarter of the spring semester

The enrolment is done by the D-BIOL study administration.
6 KP7GK. Locher, G. Schertler
KurzbeschreibungThis course will familiarize the students with techniques used for the and structural characterization of biological macromolecules. The students will carry out many of the steps involved in high resolution structure determination using X-ray crystallography as the main technique.
LernzielThe course aims at introducing the principles of protein X-ray crystallography and related modern techniques (e.g. FEL-based crystallography). Students will get an opportunity to conduct hands-on experiments and also use computational techniques.
InhaltStudents will crystallize a protein, collect X-ray diffraction data using an in-house or synchrotron X-ray source, analyze and process this data, and build an atomic model into an electron density map. They will refine this model and interpret and illustrate the determined structure. The course also includes a demonstration of the Synchrotron capabilities at the Paul Scherrer Institute (SLS).
Voraussetzungen / BesonderesThe students will be split into two groups for the practical part of the work: One group will work at ETH Honggerberg, the other at the Paul Scherrer Institute (PSI) at Villigen. All students will spend one full day at the PSI for a tour of the facilities, including a visit of the synchrotron beam lines of the Swiss Light Source SLS.