Tomaso Zambelli: Katalogdaten im Herbstsemester 2020

NameHerr Prof. Dr. Tomaso Zambelli
Inst. f. Biomedizinische Technik
ETH Zürich, GLC F 12.2
Gloriastrasse 37/ 39
8092 Zürich
Telefon+41 44 632 45 75
DepartementInformationstechnologie und Elektrotechnik

227-0393-10LBioelectronics and Biosensors Information 6 KP2V + 2UJ. Vörös, M. F. Yanik, T. Zambelli
KurzbeschreibungThe course introduces the concepts of bioelectricity and biosensing. The sources and use of electrical fields and currents in the context of biological systems and problems are discussed. The fundamental challenges of measuring biological signals are introduced. The most important biosensing techniques and their physical concepts are introduced in a quantitative fashion.
LernzielDuring this course the students will:
- learn the basic concepts in biosensing and bioelectronics
- be able to solve typical problems in biosensing and bioelectronics
- learn about the remaining challenges in this field
InhaltL1. Bioelectronics history, its applications and overview of the field
- Volta and Galvani dispute
- BMI, pacemaker, cochlear implant, retinal implant, limb replacement devices
- Fundamentals of biosensing
- Glucometer and ELISA

L2. Fundamentals of quantum and classical noise in measuring biological signals

L3. Biomeasurement techniques with photons

L4. Acoustics sensors
- Differential equation for quartz crystal resonance
- Acoustic sensors and their applications

L5. Engineering principles of optical probes for measuring and manipulating molecular and cellular processes

L6. Optical biosensors
- Differential equation for optical waveguides
- Optical sensors and their applications
- Plasmonic sensing

L7. Basic notions of molecular adsorption and electron transfer
- Quantum mechanics: Schrödinger equation energy levels from H atom to crystals, energy bands
- Electron transfer: Marcus theory, Gerischer theory

L8. Potentiometric sensors
- Fundamentals of the electrochemical cell at equilibrium (Nernst equation)
- Principles of operation of ion-selective electrodes

L9. Amperometric sensors and bioelectric potentials
- Fundamentals of the electrochemical cell with an applied overpotential to generate a faraday current
- Principles of operation of amperometric sensors
- Ion flow through a membrane (Fick equation, Nernst equation, Donnan equilibrium, Goldman equation)

L10. Channels, amplification, signal gating, and patch clamp Y4

L11. Action potentials and impulse propagation

L12. Functional electric stimulation and recording
- MEA and CMOS based recording
- Applying potential in liquid - simulation of fields and relevance to electric stimulation

L13. Neural networks memory and learning
LiteraturPlonsey and Barr, Bioelectricity: A Quantitative Approach (Third edition)
Voraussetzungen / BesonderesThe course requires an open attitude to the interdisciplinary approach of bioelectronics.
In addition, it requires undergraduate entry-level familiarity with electric & magnetic fields/forces, resistors, capacitors, electric circuits, differential equations, calculus, probability calculus, Fourier transformation & frequency domain, lenses / light propagation / refractive index, Michaelis-Menten equation, pressure, diffusion AND basic knowledge of biology and chemistry (e.g. understanding the concepts of concentration, valence, reactants-products, etc.).
227-0939-00LCell Biophysics6 KP4GT. Zambelli
KurzbeschreibungA mathematical description is derived for a variety of biological phenomena at the molecular and cellular level applying the two fundamental principles of thermodynamics (entropy maximization and Gibbs energy minimization).
LernzielEngineering uses the laws of physics to predict the behavior of a system. Biological systems are so diverse and complex prompting the question whether we can apply unifying concepts of theoretical physics coping with the multiplicity of life’s mechanisms.

Objective of this course is to show that biological phenomena despite their variety can be analytically described using only two concepts from statistical mechanics: maximization of the entropy and minimization of the Gibbs free energy.

Starting point of the course is the probability theory, which enables to derive step-by-step the two pillars of statistical mechanics: the maximization of entropy according to the Boltzmann’s law as well as the minimization of the Gibbs free energy. Then, an assortment of biological phenomena at the molecular and cellular level (e.g. cytoskeletal polymerization, action potential, photosynthesis, gene regulation, morphogen patterning) will be examined at the light of these two principles with the aim to derive a quantitative expression describing their behavior according to experimental data.

By the end of the course, students will also learn to critically evaluate the concepts of making an assumption and making an approximation.
Inhalt1. Basics of theory of probability

2. Boltzmann's law

3. Entropy maximization and Gibbs free energy minimization

4. Two-state systems and the MWC model

5. Random walks and macromolecular structures

6. Electrostatics for salty solutions

7. Elasticity: fibers and membranes

8. Diffusion and crowding: cell signaling

9. Molecular motors

10. Action potential: Hodgkin-Huxley model

11. Photosynthesis

12. Gene regulation

13. Development: Turing patterns

14. Sequences and evolution
Literatur- Statistical Mechanics: K. Dill, S. Bromberg, Molecular Driving Forces, 2nd Edition, Garland Science, 2010.

- Biophysics: R. Phillips, J. Kondev, J. Theriot, H. Garcia, Physical Biology of the Cell, 2nd Edition, Garland Science, 2012.
Voraussetzungen / BesonderesParticipants need a good command of differentiation and integration of a function with one or more variables (calculus) as well as of Newton's and Coulomb's laws (basics of mechanics and electrostatics). Notions of vectors in 2D and 3D are beneficial.

Theory and corresponding exercises are merged together during the classes.