Paola Picotti: Catalogue data in Autumn Semester 2021

Name Prof. Dr. Paola Picotti
FieldMolecular Systems Biology
Inst. f. Molekulare Systembiologie
ETH Zürich, HPM H 46
Otto-Stern-Weg 3
8093 Zürich
Telephone+41 44 633 25 58
Fax+41 44 633 12 98
RelationshipFull Professor

551-1003-00LMethods of Biological Analysis Information
Only for Biologie BSc, Programme Regulations 2013.
3 credits3GM. Badertscher, P. Picotti, K. Weis
AbstractThe course will teach the basis and typical applications of methods for the analysis of nucleic acid sequences, mass spectrometric analysis of proteins and proteomes and advanced light and fluorescent imaging methods.
ObjectiveKnowledge of the theoretical basis of the methods for nucleic acid sequence analysis, mass spectrometry based protein and proteome analysis and advanced light and fluorescent imaging methods, and an understanding of the application of these principles in experimental biology.
ContentThe course will consist of lectures covering the theoretical and technical base of the respective analytical methods and of exercises where typical applications of the methods in modern experimental biology are discussed.
Lecture notesMaterials supporting the lectures and exercises will be made available via Moodle.
551-1005-00LBioanalytics Information 4 credits4GP. Picotti, F. Allain, V. Korkhov, M. Pilhofer, R. Schlapbach, K. Weis, K. Wüthrich, further lecturers
AbstractThe course will introduce students to a selected set of laboratory techniques that are foundational to modern biological research.
ObjectiveFor each of the techniques covered in the course, the students will be able to explain:
a) the physical, chemical and biological principles underlying the technique,
b) the requirements for the sample,
c) the type of raw data collected by the technique,
d) the assumptions and auxiliarry information used in the interpretation of the data and
e) how these data can be used to answer a given biological question.
By the end of the course the students will be able to select the appropriate experimental technique to answer a given biological problem and will be able to discuss the
advantages and limitations of individual techniques as well as how different techniques can be combined to gain a more complete understanding of a given biological questions.
ContentThe course will be based on a combination of lectures, selfstudy elements and exercises.

The focus will be on the following experimental techniques:

- DNA sequencing
- chromatography
- mass-spectrometry
- UV/Vis and fluorescence spectrometry
- light microscopy
- electron microscopy
- X-ray crystallography
- NMR spectroscopy
Lecture notesThe course is supported by a Moodle page that gives access to all supporting materials necessary for the course.
Subject-specific CompetenciesConcepts and Theoriesassessed
Techniques and Technologiesassessed
Method-specific CompetenciesAnalytical Competenciesassessed
Media and Digital Technologiesfostered
Project Managementfostered
Personal CompetenciesCreative Thinkingassessed
Critical Thinkingassessed