Paola Picotti: Catalogue data in Autumn Semester 2023 |
| Name | Prof. Dr. Paola Picotti |
| Field | Molecular Systems Biology |
| Address | Inst. f. Molekulare Systembiologie ETH Zürich, HPM H 46 Otto-Stern-Weg 3 8093 Zürich SWITZERLAND |
| Telephone | +41 44 633 25 58 |
| Fax | +41 44 633 12 98 |
| picotti@imsb.biol.ethz.ch | |
| Department | Biology |
| Relationship | Full Professor |
| Number | Title | ECTS | Hours | Lecturers | ||||||||||||||||||||||||||||||||
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| 551-0352-00L | Introduction to Mass Spectrometry-Based Proteomics  Number of participants limited to 12. The enrolment is done by the D-BIOL study administration. | 6 credits | 7P | L. Gillet, P. Picotti | ||||||||||||||||||||||||||||||||
| Abstract | Protein Analysis by Mass Spectrometry The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis. | |||||||||||||||||||||||||||||||||||
| Learning objective | How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up) Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments) Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares) Perform quantitative proteomic analysis (label-free and labeled analyses) Analyze/interpret the data to find up/down regulated proteins | |||||||||||||||||||||||||||||||||||
Competencies |
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| 551-1005-00L | Bioanalytics | 4 credits | 4G | P. Picotti, F. Allain, V. Korkhov, M. Pilhofer, R. Schlapbach, K. Weis, K. Wüthrich, further lecturers | ||||||||||||||||||||||||||||||||
| Abstract | The course will introduce students to a selected set of laboratory techniques that are foundational to modern biological research. | |||||||||||||||||||||||||||||||||||
| Learning objective | For each of the techniques covered in the course, the students will be able to explain: a) the physical, chemical and biological principles underlying the technique, b) the requirements for the sample, c) the type of raw data collected by the technique, d) the assumptions and auxiliarry information used in the interpretation of the data and e) how these data can be used to answer a given biological question. By the end of the course the students will be able to select the appropriate experimental technique to answer a given biological problem and will be able to discuss the advantages and limitations of individual techniques as well as how different techniques can be combined to gain a more complete understanding of a given biological questions. | |||||||||||||||||||||||||||||||||||
| Content | The course will be based on a combination of lectures, selfstudy elements and exercises. The focus will be on the following experimental techniques: - DNA sequencing - chromatography - mass-spectrometry - UV/Vis and fluorescence spectrometry - light microscopy - electron microscopy - X-ray crystallography - NMR spectroscopy | |||||||||||||||||||||||||||||||||||
| Lecture notes | The course is supported by a Moodle page that gives access to all supporting materials necessary for the course. | |||||||||||||||||||||||||||||||||||
| Competencies |
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