Paola Picotti: Catalogue data in Spring Semester 2022 |
| Name | Prof. Dr. Paola Picotti |
| Field | Molecular Systems Biology |
| Address | Inst. f. Molekulare Systembiologie ETH Zürich, HPM H 46 Otto-Stern-Weg 3 8093 Zürich SWITZERLAND |
| Telephone | +41 44 633 25 58 |
| Fax | +41 44 633 12 98 |
| picotti@imsb.biol.ethz.ch | |
| Department | Biology |
| Relationship | Full Professor |
| Number | Title | ECTS | Hours | Lecturers | |
|---|---|---|---|---|---|
| 551-0130-00L | Fundamentals of Biology II   Registrations via myStudies until 2.2.2022 at the latest. Subsequent registrations will not be considered. | 8 credits | 8P | M. Gstaiger, N. Aceto, J. A. Antunes Pereira, M. Cangkrama, H. Gehart, Z. Kontarakis, W. Kovacs, A. Leitner, S. L. Masneuf, P. Picotti, U. Sauer, E. B. Truernit, A. Wutz, N. Zamboni | |
| Abstract | This introductory Laboratory course introduces the student to the entire range of classical and modern molecular biosciences. In the second year (Praktikum GL Bio II) the students will perform three praktikum days in: - Plant Biology - Animal Models - Genomics - Molecular Systems Biology (total of 12 experiments) Each experiment takes one full day. | ||||
| Learning objective | Introduction to theoretical and experimental biology General Praktikum-information and course material: Moodle The general Praktikum information (Assignment list, Instructions and Schedule & Performance Sheet) will also be sent to the students directly (E-mail). | ||||
| Content | The class is divided into four blocks: Cell Biology, Plant Biology, Genomics AND Molecular Systems Biology. One block lasts three weeks. Animal Models: - Tissue structure and biology - Mouse anatomy and histology - Tissue repair and cancer GENOMICS: - Chromosome preparations from mammalian cells - Genome Editing - Cancer genomics Molecular Systems Biology: - Preparation of proteomics and metabolomics samples - Analysis of proteomics and metabolomics data - Interpretation of proteomics and metabolomics data PLANT BIOLOGY: - Plants and light - Phytohormones and other growth factors - Molecular biology of systemic gene silencing | ||||
| Lecture notes | Laboratory manuals All scripts and additional course information is available on Moodle. | ||||
| Prerequisites / Notice | THE PRAKTIKUM RULES: Your attendance is obligatory and you have to attend all 12 Praktikum days. Absences are only acceptable if you are able to provide a Doctor’s certificate. The original Dr's certificate has to be given to Dr. M. Gstaiger (HPM F43) within five days of the absence of the Praktikum day. If there will be any exceptional or important situations then you should directly contact the Director of Studies of D-Biol, who will decide if you are allowed to miss a Praktikum day or not. HIGHLY IMPORTANT!! 1. Due to the increased number of students, the official Praktikum registration has to be done, using myStudies, preferably at the end of HS21 but not later than 2.2.2022. 2. Later registration is NOT possible and can NOT be accepted! 3. The course registration for FS22 is usually possible at the end of HS21 and you will obtain an E-mail from the Rectorate when the course registration using myStudies is possible. Students can register for a practice group via myStudies. As soon as the course unit is registered in myStudies, a text box appears indicating that a group can be selected. Accordingly, students can select a group in the next step. If more than 180 students register, the surplus students will be placed on a waiting list and then allocated by the course responsible. The Praktikum GL BioII FS22 will take place during the following days and therefore, you have to make sure already now that you do not have any other activities & commitments during these days: PRAKTIKUM DAYS DURING FS22 (Monday): 21.02.; 28.02.; 07.03.; 14.03.; 21.03.; 28.03.; 04.04.; 11.04.; 02.05.; 09.05.; 16.05.; 23.05.; No Praktikum during the Easter break: 18.04-29.04. | ||||
| 551-0224-00L | Advanced Proteomics For master students from the 2nd semester on, also doctoral candidates and post docs. | 4 credits | 6G | P. Picotti, L. Gillet, A. Leitner, P. Pedrioli | |
| Abstract | Goal of the course is to analyze current and newly emerging technologies and approaches in protein and proteome analysis with regard to their application in biology, biotechnology and medicine. Format: Introduction by instructor followed by discussions stimulated by reading assignments and exercises. | ||||
| Learning objective | To discuss current and newly emerging technologies and approaches in protein and proteome analysis with regard to their applications in biology, biotechnology, medicine and systems biology. | ||||
| Content | Block course teaching current methods for the acquisition and processing of proteomic datasets. | ||||
| Prerequisites / Notice | Number of people: Not exceeding 30. Students from ETHZ, Uni Zurich and University of Basel Non-ETH students must register at ETH Zurich as special students http://www.rektorat.ethz.ch/students/admission/auditors/index_EN | ||||
| 551-0324-00L | Systems Biology | 6 credits | 4V | P. Picotti, M. Claassen, U. Sauer, B. Snijder, B. Wollscheid | |
| Abstract | Introduction to experimental and computational methods of systems biology. By using baker’s yeast as a thread through the series, we focus on global methods for analysis of and interference with biological functions. Illustrative applications to other organisms will highlight medical and biotechnological aspects. | ||||
| Learning objective | - obtain an overview of global analytical methods - obtain an overview of computational methods in systems biology - understand the concepts of systems biology | ||||
| Content | Overview of global analytical methods (e.g. DNA arrays, proteomics, metabolomics, fluxes etc), global interference methods (siRNA, mutant libraries, synthetic lethality etc.) and imaging methods. Introduction to mass spectrometry and proteomics. Concepts of metabolism in microbes and higher cells. Systems biology of developmental processes. Concepts of mathematical modeling and applications of computational systems biology. | ||||
| Lecture notes | no script | ||||
| Literature | The course is not taught by a particular book, but some books are suggested for further reading: - Systems biology in Practice by Klipp, Herwig, Kowald, Wierling und Lehrach. Wiley-VCH 2005 | ||||
| 551-0352-00L | Introduction to Mass Spectrometry-Based Proteomics Number of participants limited to 12. The enrolment is done by the D-BIOL study administration. | 6 credits | 7P | L. Gillet, P. Picotti | |
| Abstract | Protein Analysis by Mass Spectrometry The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis. | ||||
| Learning objective | How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up) Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments) Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares) Perform quantitative proteomic analysis (label-free and labeled analyses) Analyze/interpret the data to find up/down regulated proteins | ||||