Paola Picotti: Catalogue data in Autumn Semester 2021 |
| Name | Prof. Dr. Paola Picotti |
| Field | Molecular Systems Biology |
| Address | Inst. f. Molekulare Systembiologie ETH Zürich, HPM H 46 Otto-Stern-Weg 3 8093 Zürich SWITZERLAND |
| Telephone | +41 44 633 25 58 |
| Fax | +41 44 633 12 98 |
| picotti@imsb.biol.ethz.ch | |
| Department | Biology |
| Relationship | Full Professor |
| Number | Title | ECTS | Hours | Lecturers | |||||||||||||||||||||||||||||
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| 551-1003-00L | Methods of Biological Analysis  Only for Biologie BSc, Programme Regulations 2013. | 3 credits | 3G | M. Badertscher, P. Picotti, K. Weis | |||||||||||||||||||||||||||||
| Abstract | The course will teach the basis and typical applications of methods for the analysis of nucleic acid sequences, mass spectrometric analysis of proteins and proteomes and advanced light and fluorescent imaging methods. | ||||||||||||||||||||||||||||||||
| Learning objective | Knowledge of the theoretical basis of the methods for nucleic acid sequence analysis, mass spectrometry based protein and proteome analysis and advanced light and fluorescent imaging methods, and an understanding of the application of these principles in experimental biology. | ||||||||||||||||||||||||||||||||
| Content | The course will consist of lectures covering the theoretical and technical base of the respective analytical methods and of exercises where typical applications of the methods in modern experimental biology are discussed. | ||||||||||||||||||||||||||||||||
| Lecture notes | Materials supporting the lectures and exercises will be made available via Moodle. | ||||||||||||||||||||||||||||||||
| 551-1005-00L | Bioanalytics | 4 credits | 4G | P. Picotti, F. Allain, V. Korkhov, M. Pilhofer, R. Schlapbach, K. Weis, K. Wüthrich, further lecturers | |||||||||||||||||||||||||||||
| Abstract | The course will introduce students to a selected set of laboratory techniques that are foundational to modern biological research. | ||||||||||||||||||||||||||||||||
| Learning objective | For each of the techniques covered in the course, the students will be able to explain: a) the physical, chemical and biological principles underlying the technique, b) the requirements for the sample, c) the type of raw data collected by the technique, d) the assumptions and auxiliarry information used in the interpretation of the data and e) how these data can be used to answer a given biological question. By the end of the course the students will be able to select the appropriate experimental technique to answer a given biological problem and will be able to discuss the advantages and limitations of individual techniques as well as how different techniques can be combined to gain a more complete understanding of a given biological questions. | ||||||||||||||||||||||||||||||||
| Content | The course will be based on a combination of lectures, selfstudy elements and exercises. The focus will be on the following experimental techniques: - DNA sequencing - chromatography - mass-spectrometry - UV/Vis and fluorescence spectrometry - light microscopy - electron microscopy - X-ray crystallography - NMR spectroscopy | ||||||||||||||||||||||||||||||||
| Lecture notes | The course is supported by a Moodle page that gives access to all supporting materials necessary for the course. | ||||||||||||||||||||||||||||||||
| Competencies |
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