Kaspar Locher: Katalogdaten im Frühjahrssemester 2022

NameHerr Prof. Dr. Kaspar Locher
LehrgebietMolekulare Membranbiologie
Adresse
Inst. f. Molekularbiol.u.Biophysik
ETH Zürich, HPK G 11
Otto-Stern-Weg 5
8093 Zürich
SWITZERLAND
Telefon+41 44 633 39 91
E-Maillocher@mol.biol.ethz.ch
URLhttp://www.locherlab.ethz.ch
DepartementBiologie
BeziehungOrdentlicher Professor

NummerTitelECTSUmfangDozierende
551-1324-00LBiochemie5 KP4GK. Locher, N. Ban, R. Glockshuber, J. Piel, E. Weber-Ban
KurzbeschreibungDie Vorlesung vermittelt zentrale Reaktionen und Stoffwechselwege der Biochemie sowie Molekularbiologie mit Betonung der chemischen und z.T. biophysikalischen Aspekte.
LernzielVerständnis der wichtigsten an zellulärer Energiegewinnung und -Speicherung beteiligten Stoffwechselvorgänge sowie ausgewählter biosynthetischer Prozesse (inkl. Zucker, Fette, Steroide etc). Verständnis der molekularen Vorgänge bei Replikation, Transkription und Translation.
Voraussetzungen / BesonderesEinige Vorlesungseinheiten werden in englischer Sprache gehalten.
551-1412-00LMolecular and Structural Biology IV: Visualizing Macromolecules by X-Ray Crystallography and EM4 KP2VN. Ban, D. Böhringer, T. Ishikawa, M. A. Leibundgut, K. Locher, M. Pilhofer, K. Wüthrich, weitere Dozierende
KurzbeschreibungThis course provides an in-depth discussion of two main methods to determine the 3D structures of macromolecules and complexes at high resolution: X-ray crystallography and cryo-electron microscopy. Both techniques result in electron density maps that are interpreted by atomic models.
LernzielStudents will obtain the theoretical background to understand structure determination techniques employed in X-ray crystallography and electron microscopy, including diffraction theory, crystal growth and analysis, reciprocal space calculations, interpretation of electron density, structure building and refinement as well as validation. The course will also provide an introduction into the use of cryo-electron tomography to visualize complex cellular substructures at sub-nanometer resolutions, effectively bridging the resolution gap between optical microscopy and single particle cryo-electron microscopy. Lectures will be complemented with practical sessions where students will have a chance to gain hands on experience with sample preparation, data processing and structure building and refinement.
Inhalt- History of Structural Molecular Biology

- X-ray diffraction from macromolecular crystals

- Data collection and statistics, phasing methods

- Crystal symmetry and space groups

- X-ray data processing

- Principle of cryo-EM for biological macromolecules I, including hardware of TEM and detectors, image formation principle (phase contrast, spherical aberration, CTF), 3D reconstruction (central-section theorem, backprojection, missing information)

- Single particle analysis, including principle (projection matching, random conical tilt, angular reconstitution)

- Tomography I, including basics and subtomogram averaging

- Tomography - recent techniques, including cryo-FIB
- EM specimen preparation (cryo, negative stain), initial EM data processing

- EM and X-ray structure building, refinement, validation and interpretation

- Model building and refinement
551-1556-00LStructure Determination by Cryo-EM: Data Processing and Analysis Belegung eingeschränkt - Details anzeigen
Findet dieses Semester nicht statt.
Number of participants limited to15



The block course will only take place with a minimum of 4 participants.

The enrolment is done by the D-BIOL study administration.
6 KP7PK. Locher
KurzbeschreibungThis course will introduce the students to high-resolution structure determination using single particle cryo-electron microscopy, one of the key techniques for determining structures of biological macromolecules
LernzielThe goal of this course is to provide the students with the opportunity to pursue the processing of cryo-EM data and to gain experience in the building and refinement of an atomic model of a protein.
InhaltThe students will receive a demonstration of sample vitrification and sample imaging using a cryo-electron microscope. The students will then use a pre-recorded data set to perform the calculations involved in determining the 3D structure of a model protein. Students will learn how to build an atomic model into their electron density maps, how to refine and analyze this model, and how to present their structural data. The following software packages will be used: Relion, Coot, Phenix, Pymol, Chimera.
Voraussetzungen / BesonderesThe students will spend the majority of the time on data processing and are therefore expected to have some basic knowledge of bash terminal (Linux) commands. Basic physics, optics and linear algebra knowledge is also helpful. By the end of the course, the students will be expected to understand concepts such as the difference between Fourier and real space, image formation, the contrast transfer function (CTF), the fast Fourier transform (FFT), and Fourier shell correlation (FSC). During the course the students will be expected to complete homework assignments. At the end of the course, the students will give an oral presentation on what they learned. After the course, the students will submit a written report prepared individually.
551-1620-00LMolecular Biology, Biophysics1 KP1KR. Glockshuber, F. Allain, N. Ban, K. Locher, E. Weber-Ban, K. Wüthrich
KurzbeschreibungThe course consists of a series of research seminars on Structural Biology and Biophysics, given by both scientists of the National Center of Competence in Research (NCCR) in Structural Biology and external speakers.
LernzielThe goal of this course is to provide doctoral and postdoctoral students with a broad overview on the most recent developments in biochemistry, structural biology and biophysics.
Voraussetzungen / BesonderesInformation on the individual seminars is provided on the following websites:
http://www.structuralbiology.unizh.ch/events005.asp
http://www.biol.ethz.ch/dbiol-cal/index