Kaspar Locher: Katalogdaten im Frühjahrssemester 2018

NameHerr Prof. Dr. Kaspar Locher
LehrgebietMolekulare Membranbiologie
Adresse
Inst. f. Molekularbiol.u.Biophysik
ETH Zürich, HPK G 11
Otto-Stern-Weg 5
8093 Zürich
SWITZERLAND
Telefon+41 44 633 39 91
E-Maillocher@mol.biol.ethz.ch
URLhttp://www.locherlab.ethz.ch
DepartementBiologie
BeziehungOrdentlicher Professor

NummerTitelECTSUmfangDozierende
551-1323-AALFundamentals of Biology II: Biochemistry and Molecular Biology
Belegung ist NUR erlaubt für MSc Studierende, die diese Lerneinheit als Auflagenfach verfügt haben.

Alle anderen Studierenden (u.a. auch Mobilitätsstudierende, Doktorierende) können diese Lerneinheit NICHT belegen.
4 KP11RK. Locher, N. Ban, R. Glockshuber, E. Weber-Ban
KurzbeschreibungThe course provides an introduction to Biochemistry / Molecular Biology with some emphasis on chemical and biophysical aspects.
LernzielTopics include the structure-function
relationship of proteins / nucleic acids, protein folding, enzymatic catalysis, cellular pathways involved in bioenergetics and the biosynthesis and breakdown of amino acids, glycans, nucleotides, fatty acids and phospholipids, and steroids. There will also be a discussion of DNA replication and repair, transcription, and translation.
Skriptnone
Literatur"Biochemistry",
Berg/Tymoczko/Stryer, 8th edition, Palgrave Macmillan, International edition
551-1412-00LMolecular and Structural Biology IV: Visualizing Macromolecules by X-ray Crystallography and EM4 KP2VN. Ban, D. Böhringer, T. Ishikawa, M. A. Leibundgut, K. Locher, M. Pilhofer, K. Wüthrich
KurzbeschreibungThis course provides an in-depth discussion of two main methods to determine the 3D structures of macromolecules and complexes at high resolution: X-ray crystallography and cryo-electron microscopy. Both techniques result in electron density maps that are interpreted by atomic models.
LernzielStudents will obtain the theoretical background to understand structure determination techniques employed in X-ray crystallography and electron microscopy, including diffraction theory, crystal growth and analysis, reciprocal space calculations, interpretation of electron density, structure building and refinement as well as validation. The course will also provide an introduction into the use of cryo-electron tomography to visualize complex cellular substructures at sub-nanometer resolutions, effectively bridging the resolution gap between optical microscopy and single particle cryo-electron microscopy. Lectures will be complemented with practical sessions where students will have a chance to gain hands on experience with sample preparation, data processing and structure building and refinement.
551-1556-00LMacromolecular Structure Determination Using Modern Methods Belegung eingeschränkt - Details anzeigen
Number of participants limited to 11 in the 3rd semester quarter of the spring semester

Number of participants limited to 12 in the 4th semester quarter of the spring semester

The enrolment is done by the D-BIOL study administration.
6 KP7GK. Locher, G. Schertler
KurzbeschreibungThis course will familiarize the students with techniques used for the and structural characterization of biological macromolecules. The students will carry out many of the steps involved in high resolution structure determination using X-ray crystallography as the main technique.
LernzielThe course aims at introducing the principles of protein X-ray crystallography and related modern techniques (e.g. FEL-based crystallography). Students will get an opportunity to conduct hands-on experiments and also use computational techniques.
InhaltStudents will crystallize a protein, collect X-ray diffraction data using an in-house or synchrotron X-ray source, analyze and process this data, and build an atomic model into an electron density map. They will refine this model and interpret and illustrate the determined structure. The course also includes a demonstration of the Synchrotron capabilities at the Paul Scherrer Institute (SLS).
Voraussetzungen / BesonderesThe students will be split into two groups for the practical part of the work: One group will work at ETH Honggerberg, the other at the Paul Scherrer Institute (PSI) at Villigen. All students will spend one full day at the PSI for a tour of the facilities, including a visit of the synchrotron beam lines of the Swiss Light Source SLS.
551-1620-00LMolecular Biology, Biophysics1 KP1KR. Glockshuber, F. Allain, N. Ban, K. Locher, E. Weber-Ban, K. Wüthrich
KurzbeschreibungThe course consists of a series of research seminars on Structural Biology and Biophysics, given by both scientists of the National Center of Competence in Research (NCCR) in Structural Biology and external speakers.
LernzielThe goal of this course is to provide doctoral and postdoctoral students with a broad overview on the most recent developments in biochemistry, structural biology and biophysics.
Voraussetzungen / BesonderesInformation on the individual seminars is provided on the following websites:
http://www.structuralbiology.unizh.ch/events005.asp
http://www.biol.ethz.ch/dbiol-cal/index