Markus Künzler: Catalogue data in Spring Semester 2023

Name Prof. Dr. Markus Künzler
Name variantsMarkus Künzler
Markus Kuenzler
Markus Kunzler
Address
Institut für Mikrobiologie
ETH Zürich, HCI F 423
Vladimir-Prelog-Weg 1-5/10
8093 Zürich
SWITZERLAND
Telephone+41 44 632 49 25
E-mailmarkus.kuenzler@micro.biol.ethz.ch
URLhttps://micro.biol.ethz.ch/research/kuenzler.html
DepartmentBiology
RelationshipAdjunct Professor

NumberTitleECTSHoursLecturers
551-0128-00LFundamentals of Biology I Restricted registration - show details
Registrations via myStudies until 28.1.2023 at the latest. Subsequent registrations will not be considered.
8 credits8PM. Gstaiger, A. Cléry, E. Dultz, C. H. Giese, R. Kroschewski, M. Künzler
AbstractThis 1st year Laboratory course introduces the students to the basic experimental techniques used in the classical and modern molecular biosciences. In the first year (Praktikum GL BioI) the students will be introduced into the basic concepts and methods of Microbiology, Biochemistry and Molecular Biology in 12 consecutive course days.
Learning objectiveIntroduction to the biology of unicellular organisms and practical experience with biochemical and molecular biology techniques.

General Praktikum-information and course material can be obtained from Moodle.

The general Praktikum information (Assignment list and Instructions) will also be sent to the students directly (E-mail).
ContentThis 1st year Laboratory course introduces the students to the entire range of classical and modern molecular biosciences. Students will participate in 12 consecutive course days. The practical will operate in two shifts on Thursday and Friday and requires in addition theoretical preparation via Moodle.

morning shift 8:00-13:00

afternoon shift 13:30-18:30

Program

Day 1: Handling and detection of microorganisms
Day 2: Morphological, biochemical and genetic differentiation of microorganisms
Day 3: Biotic interactions and genetics of bacteria
Day 4: Metabolism and physiology of bacteria
Day 5: DNA extraction, tranformation (E.coli, yeast)
Day 6: RNA extraction, Life cycle of eukaryotic cells
Day 7: Purification of TAQ polymerase from E coli by ionexchange chromatography
Day 8: Characterizing fractions of the purification of TAQ polymerase (SDS-PAGE, WB)
Day 9: mRNA splicing in yeast by RT-PCR and purified TAQ polymerase
Day 10: Affinity chromatography, protein crystallization
Day 11: Enzyme kinetics
Day 12: Protein folding, stability and structure
Lecture notesLaboratory manuals can be downloaded from Moodle
Prerequisites / NoticePLEASE NOTE THE FOLLOWING RULES

Your attendance is obligatory and you have to attend all 12 Praktikum days of GL BioI. Absences are only acceptable if you are able to provide a Doctor’s certificate. The original Dr's certificate has to be given to Dr. M. Gstaiger (HPM F43) within five days of the absence of the Praktikum day.

If there will be any exceptional or important situations then you should directly contact the Director of Studies of D-Biol, who will decide if you are allowed to miss a Praktikum day or not.

HIGHLY IMPORTANT!!

1. Due to the increased number of students, the official Praktikum registration has to be done, using myStudies, preferably at the end of HS22 but not later than January 28, 2023.

2. Later registration is NOT possible and can NOT be accepted!

3. The course registration for FS2023 is usually possible at the end of fall semester 2022 and you will obtain an E-mail from the Rectorate when the course registration using myStudies is possible.

The 12 course days of the Praktikum Grundlagen Biologie-I will take place Thursday or Friday during the spring semester 2023. Therefore, you have to make sure already now that you will not have any other activities / commitments during these days. The exact course schedule will be communicated before the beginning of the spring semester.

PRAKTIKUM DAYS FS23 (Thursdays):

23.02.; 02.03.; 09.03.; 16.03.; 23.03.; 30.03.; 20.04.; 27.04.; 04.05.; 11.05.; 25.05.; 01.06.

PRAKTIKUM DAYS FS23 (Fridays):

24.02.; 03.03.; 10.03.; 17.03.; 24.03.; 31.03.; 21.04.; 28.04.; 05.05.; 12.05.; 26.05.; 02.06.

No Praktikum during the Easter vacation: 03.04-14.04.
551-0334-00LMolecular Defense Mechanisms of Fungi Restricted registration - show details
Number of participants limited to 6.

The enrolment is done by the D-BIOL study administration.
6 credits7PM. Künzler
AbstractThe course offers an introduction into the molecular biology of fungi by participation in a current research project on Molecular Defense Mechanisms of Fungi. The performed experiments, in conjunction with accompanying seminars should enable the students to answer questions regarding central aspects of innate defense mechanisms and the life style of multicellular fungi.
Learning objectiveThe course should enable the students to answer questions regarding central aspects of innate defense mechanisms and the life style of multicellular fungi, and their experimental accessibility.
ContentExperiments include the isolation, identification and characterization of defense effector molecules from multicellular fungi. Methods include molecular genetics, biochemistry, mass spectrometry and biotoxicity assays towards different model organisms including fungi, bacteria, insects and nematodes. Experiments are supported by seminars giving an overview over Fungal Defense Mechanisms and Fungal Lifestyle.
Literaturehttp://www.micro.biol.ethz.ch/research/aebi/kuenzler/publications
551-1109-00LSeminars in Microbiology Information 0 credits2KW.‑D. Hardt, M. Künzler, J. Piel, S. Sunagawa, J. Vorholt-Zambelli
AbstractSeminars by invited speakers covering selected microbiology themes.
Learning objectiveDiscussion of selected microbiology themes presented by invited speakers.