Markus Künzler: Catalogue data in Spring Semester 2022 |
| Name | Prof. Dr. Markus Künzler |
| Name variants | Markus Künzler Markus Kuenzler Markus Kunzler |
| Address | Institut für Mikrobiologie ETH Zürich, HCI F 423 Vladimir-Prelog-Weg 1-5/10 8093 Zürich SWITZERLAND |
| Telephone | +41 44 632 49 25 |
| markus.kuenzler@micro.biol.ethz.ch | |
| URL | https://micro.biol.ethz.ch/research/kuenzler.html |
| Department | Biology |
| Relationship | Adjunct Professor |
| Number | Title | ECTS | Hours | Lecturers | |
|---|---|---|---|---|---|
| 551-0128-00L | Fundamentals of Biology I  Registrations via myStudies until 28.1.2022 at the latest. Subsequent registrations will not be considered. | 8 credits | 8P | M. Gstaiger, A. Cléry, E. Dultz, C. H. Giese, R. Kroschewski, M. Künzler | |
| Abstract | This 1st year Laboratory course introduces the students to the basic experimental techniques used in the classical and modern molecular biosciences. In the first year (Praktikum GL BioI) the students will be introduced into the basic concepts and methods of Microbiology, Biochemistry and Molecular Biology in 12 consecutive course days. | ||||
| Learning objective | Introduction to the biology of unicellular organisms and practical experience with biochemical and molecular biology techniques. General Praktikum-information and course material can be obtained from Moodle The general Praktikum information (Assignment list, Instructions and Schedule & Performance Sheet) will also be sent to the students directly (E-mail). | ||||
| Content | This 1st year Laboratory course introduces the students to the entire range of classical and modern molecular biosciences. Students will participate in 12 consecutive course days. The practical will operate in two shifts on Thursday and Friday. morning shift 8:00-13:00 afternoon shift 13:30-18:30 Program Day 1: Handling and detection of microorganisms Day 2: Morphological, biochemical and genetic differentiation of microorganisms Day 3: Biotic interactions and genetics of bacteria Day 4: Metabolism and physiology of bacteria Day 5: DNA extraction, tranformation (E.coli, yeast) Day 6: RNA extraction, Life cycle of eukaryotic cells Day 7: Purification of TAQ polymerase from E coli by ionexchange chromatography Day 8: Characterizing fractions of the purification of TAQ polymerase (SDS-PAGE, WB) Day 9: mRNA splicing in yeast by RT-PCR and purified TAQ polymerase Day 10: Affinity chromatography, protein crystallization and structure Day 11: Protein folding, protein stability and Enzyme kinetics Day 12: Protein folding, protein stability and Enzyme kinetics | ||||
| Lecture notes | Laboratory manuals can be downloaded from Moodle | ||||
| Prerequisites / Notice | PLEASE NOTE THE FOLLOWING RULES Your attendance is obligatory and you have to attend all 12 Praktikum days of GL BioI. Absences are only acceptable if you are able to provide a Doctor’s certificate. The original Dr's certificate has to be given to Dr. M. Gstaiger (HPM F43) within five days of the absence of the Praktikum day. If there will be any exceptional or important situations then you should directly contact the Director of Studies of D-Biol, who will decide if you are allowed to miss a Praktikum day or not. HIGHLY IMPORTANT!! 1. Due to the increased number of students, the official Praktikum registration has to be done, using myStudies, preferably at the end of HS21 but not later than January 28, 2022. 2. Later registration is NOT possible and can NOT be accepted! 3. The course registration for FS2022 is usually possible at the end of fall semester 2021 and you will obtain an E-mail from the Rectorate when the course registration using myStudies is possible. The 12 course days of the Praktikum Grundlagen Biologie-I will take place Thursday or Friday during the spring semester 2022. Therefore, you have to make sure already now that you will not have any other activities / commitments during these days. The exact course schedule will be communicated before the beginning of the spring semester. PRAKTIKUM DAYS FS22 (Thursdays): 24.02.; 03.03.; 10.03.; 17.03.; 24.03.; 31.03.; 07.04.; 28.04.; 5.05.; 12.05.; 19.05.; 02.06. PRAKTIKUM DAYS FS22 (Fridays): 25.02.; 04.03.; 11.03.; 18.03.; 25.03.; 1.04.; 8.04.; 29.04.; 06.05.; 13.05.; 20.05.; 03.06. No Praktikum during the Easter vacation: 11.04-22.04. | ||||
| 551-0334-00L | Molecular Defense Mechanisms of Fungi Number of participants limited to 6. The enrolment is done by the D-BIOL study administration. | 6 credits | 7P | M. Künzler | |
| Abstract | The course offers an introduction into the molecular biology of fungi by participation in a current research project on Molecular Defense Mechanisms of Fungi. The performed experiments, in conjunction with accompanying seminars should enable the students to answer questions regarding central aspects of innate defense mechanisms and the life style of multicellular fungi. | ||||
| Learning objective | The course should enable the students to answer questions regarding central aspects of innate defense mechanisms and the life style of multicellular fungi, and their experimental accessibility. | ||||
| Content | Experiments include the isolation, identification and characterization of defense effector molecules from multicellular fungi. Methods include molecular genetics, biochemistry, mass spectrometry and biotoxicity assays towards different model organisms including fungi, bacteria, insects and nematodes. Experiments are supported by seminars giving an overview over Fungal Defense Mechanisms and Fungal Lifestyle. | ||||
| Literature | http://www.micro.biol.ethz.ch/research/aebi/kuenzler/publications | ||||
| 551-1109-00L | Seminars in Microbiology  | 0 credits | 2K | W.‑D. Hardt, M. Künzler, J. Piel, S. Sunagawa, J. Vorholt-Zambelli | |
| Abstract | Seminars by invited speakers covering selected microbiology themes. | ||||
| Learning objective | Discussion of selected microbiology themes presented by invited speakers. | ||||