Manfred Kopf: Katalogdaten im Frühjahrssemester 2023 |
| Name | Herr Prof. Dr. Manfred Kopf |
| Lehrgebiet | Molekulare Biomedizin |
| Adresse | Inst. f. Molecular Health Sciences ETH Zürich, HPL G 12 Otto-Stern-Weg 7 8093 Zürich SWITZERLAND |
| Telefon | +41 44 633 64 70 |
| Fax | +41 44 633 13 50 |
| manfred.kopf@biol.ethz.ch | |
| URL | https://mhs.biol.ethz.ch/research/kopf.html |
| Departement | Biologie |
| Beziehung | Ordentlicher Professor |
| Nummer | Titel | ECTS | Umfang | Dozierende | ||||||||||||||||||||||||||||||||||||||||||||||||||
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| 551-0318-00L | Immunology II | 3 KP | 2V | A. Oxenius, M. Kopf, S. R. Leibundgut, E. Slack, weitere Dozierende | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Einführung in die zellulären und molekularen Grundlagen des Immunsystems und die Immunreaktionen gegen verschiedene Pathogene, Tumore, Transplantate, und körpereigene Strukturen (Autoimmunität) | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lernziel | Die Vorlesung soll ein grundlegendes Verständnis vermitteln über: - die Interaktion der verschiedenen Immunzellen auf zellulärer und molekularer Ebene? - Erkennung und Abwehr ausgewählter Viren, Bakterien, und Parasiten. - Abwehr von Tumoren. - Mechanismen der Toleranz für körpereigene Moleküle. - Funktion des Immunsystems im Darm und warum kommensale Bakterien keine Immunantwort auslösen. - Immunpathologie und entzündliche Erkrankungen. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Inhalt | Ziel dieser Vorlesung ist das Verständnis: > Wie Pathogene vom unspezifischen Immunystem erkannt werden > Wie Pathogene vom Immunsystem bekämpft werden > Immunantworten der Haut, Lung, und Darms > Tumorimmunologie > Migration von Immunzellen > Toleranz und Autoimmunität > das Gedächtnis von T Zellen | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Skript | Die Vorlesungsunterlagen der Dozenten sind verfügbar in Moodle | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literatur | Empfohlen: Kuby Immunology (Freeman) | |||||||||||||||||||||||||||||||||||||||||||||||||||||
Kompetenzen |
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| 551-0396-01L | Immunology I  Number of participants limited to 30. Prerequisites: Attendance of the concept courses Immunology I (551-0317-00L) and Immunology II (551-0318-00L) The enrolment is done by the D-BIOL study administration. | 6 KP | 7P | A. Oxenius, B. Becher, M. Kopf, B. Ludewig, C. Münz, R. Spörri, M. van den Broek | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Dieser Blockkurs in Immunologie vermittelt einen breiten Einblick und eine Einführung in praktisches Immunologisches Arbeiten sowie theoretische Vertiefungen in ausgewählten Gebieten der Immunologie. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lernziel | Das Ziel des Blockkurses ist das Erlernen verschiedener immunologischer Techniken und umfasst die experimentelle Durchführung als auch Analyse und Interpretation der experimentellen Daten. Begleitet wird der praktische Teil von vertiefenden Vorlesungen in ausgewählten Gebieten der Immunologie, welche auf dem Inhalt des Immunologie-Konzeptkurses basieren. Selbständiges Erarbeiten und Präsentieren von Publikationen durch die Studenten bietet Grundlage für wissenschafltiche Diskussionen. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Inhalt | Praktische Arbeiten: Zellkultur, Isolation hämatopoietische Stammzellen und Differenzierung von Makrophagen und dendritischen Zellen, Aktivierung und Zytokinproduktion durch Makrophagen und dendritische Zellen, 51Cr release assay, VSV Neutralisationsassay, Durchflusszytometrie, Proliferationsexperimente, SEREX, Intrazelluläres Zytokinstaining, Immunhistologie und Fluoreszenzmikroskopie, MACS, Zytokin-Bioassays, Phagozytose, Proteosomale Prozessierung Vertiefende Vorlesungen: Immune responses to pathogens, Vaccination and B cells, Tolerance & Autoimmunity, Antigen processing & presentation, Pattern recognition, NK cells, Generation of (TCR) tg or ko mice, Antigen screening and definition | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Skript | Ein Skript wird vor Kursbeginn online abrufbar sein (link wird im Immunologie-konzeptkurs bekannt gegeben, 551-0318-00L). | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Voraussetzungen / Besonderes | Voraussetzung für die Anmeldung zum Kurs ist der Besuch der Immunologie-Konzeptvorlesungen 551-0317-00L und 551-0318-00L. Leistungskontrolle erfolgt individuell durch die beteiligten Dozenten. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kompetenzen |
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| 551-0509-00L | Current Immunological Research in Zurich | 0 KP | 1K | R. Spörri, C. Halin Winter, W.‑D. Hardt, M. Kopf, S. R. Leibundgut, A. Oxenius, Uni-Dozierende | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | This monthly meeting is a platform for Zurich-based immunology research groups to present and discuss their ongoing research projects. At each meeting three PhD students or Postdocs from the participating research groups present an ongoing research project in a 30 min seminar followed by a plenary discussion. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lernziel | The aim of this monthly meeting is to provide further education for master and doctoral students as well as Postdocs in diverse topics of immunology and to give an insight in the related research. Furthermore, this platform fosters the establishment of science- and technology-based interactions between the participating research groups. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Inhalt | Presentation and discussion of current research projects carried out by various immunology-oriented research groups in Zurich. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Skript | none | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| 551-1100-00L | Infectious Agents: From Molecular Biology to Disease Number of participants limited to 22. Requires application until 2 weeks before the start of the semester; selected applicants will be notified one week before the first week of lectures. (if you missed the deadline, please come to the first date to see, if there are any slots left) | 4 KP | 2S | W.‑D. Hardt, A. B. Hehl, M. Kopf, S. R. Leibundgut, C. Münz, A. Oxenius, P. Sander, weitere Dozierende | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Literature seminar for students at the masters level and PhD students. Introduction to the current research topics in infectious diseases; Introduction to key pathogens which are studied as model organisms in this field; Overview over key research groups in the field of infectious diseases in Zürich. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lernziel | Working with the current research literature. Getting to know the key pathogens serving as model organisms and the research technologies currently used in infection biology. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Inhalt | for each model pathogen (or key technology): 1. introduction to the pathogen 2. Discussion of one current research paper. The paper will be provided by the respective supervisor. He/she will give advice (if required) and guide the respective literature discussion. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Skript | Teachers will provide the research papers to be discussed. Students will prepare handouts for the rest of the group for their assigned seminar. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literatur | Teachers will provide the research papers to be discussed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Voraussetzungen / Besonderes | Restricted to max 22 students. Please sign up until two weeks before the beginning of the semester via e-mail to micro_secr@micro.biol.ethz.ch and include the following information: 551-1100-00L; your name, your e-mail address, university/eth, students (specialization, semester), PhD students (research group, member of a PhD program? which program?). The 22 students admitted to this seminar will be selected and informed by e-mail in the week befor the beginning of the semester by W.-D. Hardt. The first seminar date will serve to form groups of students and assign a paper to each group. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kompetenzen |
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| 551-1118-00L | Cutting Edge Topics: Immunology and Infection Biology II | 2 KP | 2S | A. Oxenius, B. Becher, C. Halin Winter, N. C. Joller, M. Kopf, S. R. Leibundgut, C. Münz, F. Sallusto, C. Schneider, R. Spörri, M. van den Broek, Uni-Dozierende | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | Wöchentliches Seminar über aktuelle Themen der Immunologie und Infektionsbiologie. International renommierte Experten referieren über ihre aktuellen Forschungsresultate mit anschliessender offener Diskussion. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lernziel | Wöchentliches Seminar über aktuelle Themen der Immunologie und Infektionsbiologie. International renommierte Experten referieren über ihre aktuellen Forschungsresultate mit anschliessender offener Diskussion. Ziel der Veranstaltung ist die Konfrontation von Studenten und Doktoranden mit aktuellen Forschungsthemen und mit wissenschaftlicher Vortragsform. Studenten und Doktoranden wird die Gelegenheit geboten, sich mit diversen Themen vertieft auseinander zu setzen, welche oft in den Konzeptkursen nur knapp präsentiert werden und mit Experten auf dem Gebiet zu diskutieren. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Inhalt | Immunologie und Infektionsbiologie. Die speziellen Themen variieren jedes Semester und hängen von den eingeladenen Experten ab. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| 551-1518-00L | Molecular Mechanisms of Health and Disease | 2 KP | 2G | S. Werner, N. Aceto, M. Kopf, A. Oxenius | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | The course builds on the Fundamentals in Biology courses that are taught during the first three semesters. Students will be introduced into molecular disease mechanisms with a focus on the immune system and its role in health and disease and on cancer biology. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lernziel | To understand the fundamental developments and functions of immune defense in multicellular organisms. To understand the principles of physical barriers, mechanisms of local and systemic defense pathways, migration and communication of and in between immune cells, the fundamental principles of self versus non-self recognition To understand the molecular and functional differences between a normal cell and a cancer cell To understand the molecular mechanisms underlying cancer development and progression to metastasis, including the role of immune cells, environmental toxins and cancer-promoting viruses To understand how vulnerabilities of cancer cells are exploited for modern cancer therapy | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Skript | Powerpoint Slides of the Course | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literatur | Cancer chapter of "Alberts, Molecular Biology of the Cell, 6th edition" | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kompetenzen |
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| 551-1700-00L | Introduction to Flow Cytometry | 2 KP | 1V | F. Mair, M. Kisielow, M. Kopf | ||||||||||||||||||||||||||||||||||||||||||||||||||
| Kurzbeschreibung | This course provides a broad introduction to flow cytometry. We will cover the working principles of cytometry instrumentation, and essential aspects of experimental design and data analysis. Fluorescence-based cytometry (conventional and full spectrum) as well as mass cytometry will be covered. The format is a lecture course enriched by a visit to the ETH Flow Cytometry Core Facility. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lernziel | The objective of this course is to provide participants with a strong understanding of the basic principles underlying flow cytometry, equipping them to be self-sufficient for the systematic design, execution and analysis of cytometry experiments. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Inhalt | The course is centered on explaining the working principles of cytometry instrumentation (conventional, full spectrum and mass cytometry) and associated data analysis. In detail, we will cover the following topics: - Components of Flow Cytometry Instrumentation - Signal processing - Fluorochromes, compensation, and spectral unmixing - Visualization, gating and analysis of data - Fluorescent panel design - Cytometry assays: immunophenotyping, cell signaling, etc. - Cell sorting - Mass cytometry - Computational data analysis techniques | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Skript | Updated handouts will be provided during the class. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literatur | The lecture will reference key literature on cytometry instrumentation, experimental design, immunophenotyping and other applications. For general reading, we recommend the following: - Maciorowski et al, Basic Multicolor Flow Cytomety, Current Protocols in Immunology 2017: https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpim.26 - Cossarizza et al, Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition), European Journal of Immunology 2021: https://onlinelibrary.wiley.com/doi/10.1002/eji.202170126 - Liechti et al, An updated guide for the perplexed: cytometry in the high-dimensional era, Nature Immunology 2021: https://www.nature.com/articles/s41590-021-01006-z | |||||||||||||||||||||||||||||||||||||||||||||||||||||