Frédéric Allain: Katalogdaten im Frühjahrssemester 2023

Name	Herr Prof. Dr. Frédéric Allain
Lehrgebiet	Biomolekulare NMR
Adresse	Institut für Biochemie ETH Zürich, HPP L 14.1 Hönggerbergring 64 8093 Zürich SWITZERLAND
Telefon	+41 44 633 39 40
Fax	+41 44 633 12 94
E-Mail	allain@bc.biol.ethz.ch
Departement	Biologie
Beziehung	Ordentlicher Professor

Nummer	Titel	ECTS	Umfang	Dozierende
551-0126-00L	Grundlagen der Biologie II: Zellen	6 KP	5G	K. Weis, F. Allain, Y. Barral, W.‑D. Hardt, U. Kutay, M. Peter, I. Zemp
Kurzbeschreibung	Die Vorlesung vermittelt eine Einführung in die Funktion und Regulation von Zellen.
Lernziel	Einführung in die Funktion und Regulation von Zellen
Inhalt	Die Lehrveranstaltung vermittelt ein grundlegendes Verständnis von der Struktur, Organisation, Funktion und Regulation der Zelle. Die Vorlesung ist in zwei Hauptteile gegliedert: Teil 1: Zellbiologie der Prokaryonten, Evolution, Populationen Dieser Abschnitt erläutert die generellen Prinzipien des Aufbaus und der Regulation von prokaryontischen Zellen, und erklärt die Genetik und Evolution von Bakterien. Teil II: Vereinheitlichende Konzepte in Eukarya Dieser Vorlesungsteil gibt eine breite Einführung in die generelle Struktur von eukaryontischer Zellen, und vermittelt wichtige Konzepte, die den intrazelluläre Aufbau und Transport und der Regulation der Genexpression in Eukaryonten betreffen.
Skript	Die neu konzipierte Vorlesung wird durch Skripte unterstützt.
Literatur	Die Vorlesung wird durch Skripte unterstützt. Ausserdem kann das Lehrbuch "Molecular Biology of the Cell", Alberts et al. 6th edition, Taylor und Francis, und "Brock Biology of Microorganisms", Madigan et al. 15th edition, Pearson, als Unterstützung für den Vortrag verwendet werden. "
551-0434-00L	NMR Spectroscopy in Biology Number of participants limited to 6. The enrolment is done by the D-BIOL study administration.	6 KP	7P	F. Allain, A. D. Gossert, K. Wüthrich
Kurzbeschreibung	In this block course, students actively participate in ongoing research projects in the research groups of Profs. Allain, Wüthrich and Dr. Gossert. The students will be tutored in their experimental work by experienced postdoc students. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as exercises and literature work.
Lernziel	The course provides first "hands on" insight into applications of NMR spectroscopy in biological sciences. The course should enable the students to understand the potential and limitations of NMR applied to biological problems.
Inhalt	The topics include studies of proteins, RNA and protein-RNA interactions, Participation in one of the following projects will be possible: - NMR of RNA - NMR of several protein-RNA complexes (hnRNPF, nPTB, SR proteins) - NMR studies of protein-ligand interactions - dynamics of protein-RNA complexes - Segmental isotopic labeling to study multidomain proteins - NMR Methods Development
Skript	No script
Literatur	Lists of individual reading assignments will be handed out.
551-1312-00L	RNA-Biology II Number of participants limited to 14. The enrolment is done by the D-BIOL study administration.	6 KP	7P	S. Jonas, F. Allain, J. Corn, U. Kutay, O. Voinnet
Kurzbeschreibung	Introduction to the diversity of current RNA-research at all levels from structural biology to systems biology using mainly model systems like S. cerevisiae (yeast), mammalian cells.
Lernziel	The students will obtain an overview about the diversity of current RNA-research. They will learn to design experiments and use techniques necessary to analyze different aspects of RNA biology. Through lectures and literature seminars, they will learn about the burning questions of RNA research and discuss approaches to address these questions experimentally. In practical lab projects the students will work in one of the participating laboratories. Finally, they will learn how to present and discuss their data in an appropriate manner. Student assessment is a graded semester performance based on individual performance in the laboratory, the written exam and the project presentation.
Skript	Relevant material from the lectures will be made available during the course via the corresponding Moodle page.
Literatur	Documentation and recommended literature will be provided at the beginning and during the course.
551-1414-00L	Molecular and Structural Biology V: Studying Macromolecules by NMR and EPR	4 KP	2V	F. Allain, A. D. Gossert, G. Jeschke, K. Wüthrich
Kurzbeschreibung	The course provides an overview of experimental methods for studying function and structure of macromolecules at atomic resolution in solution. The two main methods used are Nuclear Magnetic Resonance (NMR) spectroscopy and Electron Paramagnetic Resonance (EPR) spectroscopy.
Lernziel	Insight into the methodology, areas of application and limitations of these two methods for studying biological macromolecules. Practical exercises with spectra to have hands on understanding of the methodology.
Inhalt	Part I: Historical overview of structural biology. Part II: Basic concepts of NMR and initial examples of applications. 2D NMR and isotope labeling for studying protein function and molecular interactions at atomic level. Studies of dynamic processes of proteins in solution. Approaches to study large particles. Methods for determination of protein structures in solution. Part III: NMR methods for structurally characterizing RNA and protein-RNA complexes. Part IV: EPR of biomolecules
Literatur	1) Wüthrich, K. NMR of Proteins and Nucleic Acids, Wiley-Interscience. 2) Dominguez et al, Prog Nucl Magn Reson Spectrosc. 2011 Feb;58(1-2):1-61. 3) Duss O et al, Methods Enzymol. 2015;558:279-331.