Eilika Weber-Ban: Catalogue data in Autumn Semester 2019

Name	Prof. Dr. Eilika Weber-Ban
Address	Inst. f. Molekularbiol.u.Biophysik ETH Zürich, HPK E 19 Otto-Stern-Weg 5 8093 Zürich SWITZERLAND
Telephone	+41 44 633 36 78
E-mail	eilika@mol.biol.ethz.ch
URL	https://weber-ban-lab.ethz.ch/
Department	Biology
Relationship	Adjunct Professor

Number	Title	ECTS	Hours	Lecturers
551-0307-00L	Molecular and Structural Biology I: Protein Structure and Function D-BIOL students are obliged to take part I and part II (next semester) as a two-semester course	3 credits	2V	R. Glockshuber, K. Locher, E. Weber-Ban
Abstract	Biophysics of protein folding, membrane proteins and biophysics of membranes, enzymatic catalysis, catalytic RNA and RNAi, current topics in protein biophysics and structural biology.
Learning objective	Understanding of structure-function relationships in proteins and in protein folding, detailed understanding of biophysics and physical methods as well as modern methods for protein purification and microanalytics.
Lecture notes	Scripts on the individual topics can be found under http://www.mol.biol.ethz.ch/teaching.
Literature	Basics: - Creighton, T.E., Proteins, Freeman, (1993) - Fersht, A., Enzyme, Structure and Mechanism in Protein Science (1999), Freeman. - Berg, Tymoczko, Stryer: Biochemistry (5th edition), Freeman (2001). Current topics: References will be given during the lectures. .
551-0438-00L	Protein Folding, Assembly and Degradation Number of participants limited to 14. The enrolment is done by the D-BIOL study administration.	6 credits	7G	R. Glockshuber, E. Weber-Ban
Abstract	Students will carry out defined research projects related to the current research topics of the groups of Prof. Glockshuber and Prof. Weber-Ban. The topics include mechanistic studies on the assembly of adhesive pili from pathogenic bacteria, disulfide bond formation in the bacterial periplasm, ATP-dependent chaperone-protease complexes and formation of amyloid deposits in Alzheimer's disese.
Learning objective	The course should enable the students to understand and apply biophysical methods, in particular kinetic and spectroscopic methods, to unravel the mechanism of complex reactions of biological macromolecules and assemblies in a quantitative manner.
Content	The students will be tutored in their experimental work by doctoral or postdoctoral students from the Glockshuber or Weber-Ban group. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as excercises on the numeric evaluation of biophysical data, and literature work. Participation in one of the following projects will be possible: Projects of the Glockshuber group: - Purification, biophysical characterization and structure determiation of enzymes required for disulfide bond formation in the periplasm of Gram-negative bacteria. - Mechanistic studies on the assembly of type 1 pili from pathogenic Escherichia coli strains. In vitro reconstitution of pilus assembly from all purified components. Characterization of folding, stability and assembly behaviour of individual pilus subunits. - Identification of intermediates in the aggregation of the human Abeta peptide Experimental work on these projects involves - Molecular cloning, recombinant protein production in E. coli and protein purification - Protein crystallization - Thermodynamic and kinetic characterization of conformational changes in proteins and protein-ligand interactions by fluorescence and circular dischoism spectroscopy - Analysis of rapid reactions by stopped-flow fluorescence - Negative-stain electron microscopy - Light scattering Projects of the Weber-Ban group: - Generation and purification of site-directed variants of the E. coli ClpA/P protease and chaperone-proteasome complexes from other organisms, their biophysical characterization, including rapid kinetics by stopped-flow methods, ATPase activity measurtements, negative-stain electron microscopy and light scattering
Lecture notes	No script
Literature	Literature related to the individual projects will be provided on the first day of the course.
Prerequisites / Notice	Attendance of the concept course "Biomolecular Structure and Mechanism I: Protein Structure and Function" (551-0307-00L) in the autumn semester is highly recommended for acquiring the theoretical background to this block course.
551-1323-AAL	Fundamentals of Biology II: Biochemistry and Molecular Biology Enrolment ONLY for MSc students with a decree declaring this course unit as an additional admission requirement. Any other students (e.g. incoming exchange students, doctoral students) CANNOT enrol for this course unit.	4 credits	11R	K. Locher, N. Ban, R. Glockshuber, E. Weber-Ban
Abstract	The course provides an introduction to Biochemistry / Molecular Biology with some emphasis on chemical and biophysical aspects.
Learning objective	Topics include the structure-function relationship of proteins / nucleic acids, protein folding, enzymatic catalysis, cellular pathways involved in bioenergetics and the biosynthesis and breakdown of amino acids, glycans, nucleotides, fatty acids and phospholipids, and steroids. There will also be a discussion of DNA replication and repair, transcription, and translation.
Lecture notes	none
Literature	"Biochemistry", Berg/Tymoczko/Stryer, 8th edition, Palgrave Macmillan, International edition
551-1323-00L	Fundamentals of Biology II: Biochemistry and Molecular Biology	4 credits	4G	K. Locher, N. Ban, R. Glockshuber, E. Weber-Ban
Abstract	The course provides an introduction to Biochemistry / Molecular Biology with some emphasis on chemical and biophysical aspects.
Learning objective	Topics include the structure-function relationship of proteins / nucleic acids, protein folding, enzymatic catalysis, cellular pathways involved in bioenergetics and the biosynthesis and breakdown of amino acids, glycans, nucleotides, fatty acids and phospholipids, and steroids. There will also be a discussion of DNA replication and repair, transcription, and translation.
Lecture notes	none
Literature	mandatory: "Biochemistry", Autoren: Berg/Tymoczko/Stryer, Palgrave Macmillan, International edition (the English version will be preordered at the Polybuchhandlung)
Prerequisites / Notice	Some of the lectures are given in the English language.
551-1411-00L	Molecular and Structural Biology III: Current Topics The course will only take place with a minimum of 6 participants.	2 credits	1V	K. Locher, F. Allain, N. Ban, R. Glockshuber, M. Pilhofer, E. Weber-Ban
Abstract	The course discusses current topics and cutting edge research in the structural, molecular, and biochemical study of cellular macromolecules. Student participation is an essential component of the course and will contribute to the exam grade
Learning objective	The goal is to discuss cutting edge research in the structural, molecular, and biochemical study of cellular macromolecules. Students will also have an opportunity to present and discuss recent breakthroughs relevant to the research fields presented by the faculty teaching the course (see http://www.mol.biol.ethz.ch/research.html for details on the topics).
Literature	Will be distributed by the instructors
551-1619-00L	Structural Biology	1 credit	1K	R. Glockshuber, F. Allain, N. Ban, K. Locher, M. Pilhofer, E. Weber-Ban, K. Wüthrich
Abstract	The course consists of a series of research seminars on Structural Biology, Biochemistry and Biophysics, given by both scientists of the National Center of Competence in Research (NCCR) in Structural Biology and external speakers. Information on the individual seminars is provided on the following websites: http://www.structuralbiology.uzh.ch/educ002.asp http://www.biol.ethz.ch/dbiol-cal/index
Learning objective	The goal of this course is to provide doctoral and postdoctoral students with a broad overview on the most recent developments in biochemistry, structural biology and biophysics.