Martin Held: Katalogdaten im Frühjahrssemester 2018

NameHerr Dr. Martin Held
Adresse
Bioverfahrenstechnik, Panke
ETH Zürich, BSS G 43.2
Klingelbergstrasse 48
4056 Basel
SWITZERLAND
Telefon+41 61 387 32 47
E-Mailmartin.held@bsse.ethz.ch
DepartementBiosysteme
BeziehungDozent

NummerTitelECTSUmfangDozierende
636-0204-00LLab Course: Microbial Biotechnology Belegung eingeschränkt - Details anzeigen
Only for Biotechnology MSc, Programme Regulations 2017.
2 KP5PM. Held
KurzbeschreibungStudents will learn the foundations of monoseptic working practice and create and screen microbial libraries for identification of strains expressing different fluorescent protein (XFP) levels
LernzielStudents will learn the foundations of monoseptic working practice and create and screen microbial libraries for identification of strains expressing different fluorescent protein (XFP) levels
InhaltBlock A: Handling and preparation and of microbial libraries
D1: Introduction to microbiological cultures and monoseptic working techniques.
D2: Plasmid-based expression systems and variation of XFP synthesis levels via site-directed RBS mutagenesis.
Block B: Library screening
D3: In vivo screening for XFP expression levels.
D4: Analysis of XFP levels via SDS-PAGE analysis. RBS-sequencing.
Block C: Hit recovery and validation
D5: In silico analysis of RBS variants.
D6: Cellular XFP content for selected variants at different culture conditions.
Block D: Data analysis and presentation
D7: Protein expression analysis. Q&A for reports and presentations.
D8: Final presentations and wrap-up.
SkriptMaterial will be provided during the course.
Literatur(1) Reetz MT, Kahakeaw D, and Lohmer R. "Addressing the numbers problem in directed evolution." ChemBioChem 2008
(2) Jeschek M, Gerngross D, and Panke S. “Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort.” Nat. Commun. 2016
(3) Salis HM. “The ribosome binding site calculator.” Methods Enzymol. 2011
(4) Nienhaus G, Nienhaus K, and Wiedenmann J. "Structure–Function Relationships in Fluorescent Marker Proteins of the Green Fluorescent Protein Family." Fluorescent Proteins I. Springer Berlin Heidelberg, 2011

General introduction to microbiology:

(5) Schlegel HG, and Zaborosch C. “General Microbiology.” Cambridge University Press 1993
(6) Pirt JS. “Principles of microbe and cell cultivation.” Blackwell Scientific Publications 1975