Donald Hilvert: Catalogue data in Autumn Semester 2018

Award: The Golden Owl
Name Prof. em. Dr. Donald Hilvert
FieldOrganische Chemie
Address
Dep. Chemie und Angew. Biowiss.
ETH Zürich, HCI H 327
Vladimir-Prelog-Weg 1-5/10
8093 Zürich
SWITZERLAND
Telephone+41 44 632 31 76
E-maildonald.hilvert@org.chem.ethz.ch
DepartmentChemistry and Applied Biosciences
RelationshipProfessor emeritus

NumberTitleECTSHoursLecturers
529-0290-00LOrganic Chemistry (Seminar) Restricted registration - show details 0 credits2SE. M. Carreira, J. W. Bode, P. S. Dittrich, D. Hilvert, H. Wennemers, R. Zenobi
Abstract
Objective
529-0299-00LOrganic Chemistry0 credits1.5KJ. W. Bode, E. M. Carreira, P. Chen, P. S. Dittrich, D. Hilvert, H. Wennemers, R. Zenobi
Abstract
Objective
529-0731-00LNucleic Acids and Carbohydrates6 credits3GD. Hilvert, P. A. Kast, S. J. Sturla, H. Wennemers
AbstractStructure, function and chemistry of nucleic acids and carbohydrates. DNA/RNA structure and synthesis; recombinant DNA technology and PCR; DNA arrays and genomics; antisense approach and RNAi; polymerases and transcription factors; catalytic RNA; DNA damage and repair; carbohydrate structure and synthesis; carbohydrate arrays; cell surface engineering; carbohydrate vaccines
ObjectiveStructure, function and chemistry of nucleic acids and carbohydrates. DNA/RNA structure and synthesis; recombinant DNA technology and PCR; DNA arrays and genomics; antisense approach and RNAi; polymerases and transcription factors; catalytic RNA; DNA damage and repair; carbohydrate structure and synthesis; carbohydrate arrays; cell surface engineering; carbohydrate vaccines
ContentStructure, function and chemistry of nucleic acids and carbohydrates. DNA/RNA structure and synthesis; recombinant DNA technology and PCR; DNA arrays and genomics; antisense approach and RNAi; polymerases and transcription factors; catalytic RNA; DNA damage and repair; carbohydrate structure and synthesis; carbohydrate arrays; cell surface engineering; carbohydrate vaccines
Lecture notesNo script; illustrations from the original literature relevant to the individual lectures will be provided weekly (typically as handouts downloadable from the Moodle server).
LiteratureMainly based on original literature, a detailed list will be distributed during the lecture
529-0733-00LEnzymes
Only for Chemistry MSc, Programme Regulations 2005.
7 credits3GD. Hilvert
AbstractPrinciples of enzymatic catalysis, enzyme kinetics, mechanisms of enzyme-catalyzed reactions (group transfer reactions, carbon-carbon bond formation, eliminations, isomerisations and rearrangements), cofactor chemistry, enzymes in organic synthesis and the biosynthesis of natural products, catalytic antibodies.
ObjectiveOverview of enzymes, enzyme-catalyzed reactions and metabolic processes.
ContentPrinciples of enzymatic catalysis, enzyme kinetics, mechanisms of enzyme catalyzed reactions (group transfer reactions, carbon-carbon bond formation, eliminations, isomerisations and rearrangements), cofactor chemistry, enzymes in organic synthesis and the biosynthesis of natural products, catalytic antibodies.
Lecture notesA script will not be handed out.
LiteratureGeneral:
T. Bugg, An Introduction to Enzyme and Coenzyme Chemistry, Blackwell Science Ltd., Oxford, 1997.

In addition, citations from the original literature relevant to the individual lectures will be assigned weekly.
529-0733-01LEnzymes6 credits3GD. Hilvert
AbstractPrinciples of enzymatic catalysis, enzyme kinetics, mechanisms of enzyme-catalyzed reactions (group transfer reactions, carbon-carbon bond formation, eliminations, isomerisations and rearrangements), cofactor chemistry, enzymes in organic synthesis and the biosynthesis of natural products, catalytic antibodies.
ObjectiveOverview of enzymes, enzyme-catalyzed reactions and metabolic processes.
ContentPrinciples of enzymatic catalysis, enzyme kinetics, mechanisms of enzyme catalyzed reactions (group transfer reactions, carbon-carbon bond formation, eliminations, isomerisations and rearrangements), cofactor chemistry, enzymes in organic synthesis and the biosynthesis of natural products, catalytic antibodies.
Lecture notesA script will not be handed out.
LiteratureGeneral:
T. Bugg, An Introduction to Enzyme and Coenzyme Chemistry, Blackwell Science Ltd., Oxford, 1997.

In addition, citations from the original literature relevant to the individual lectures will be assigned weekly.
529-0739-00LBiological Chemistry A: Technologies for Directed Evolution of Enzymes Restricted registration - show details
Only for Chemistry MSc, Programme Regulations 2005.

Advanced laboratory course or internship depending on lab course Biological Chemistry B.
Candidates must inquire with P. Kast no later than September 1st whether course will take place (no self-enrollment)

Further information to registration and work hours: Link
16 credits16PP. A. Kast, D. Hilvert
AbstractDuring this semester course, methodologies will be taught for biological-chemical enzyme evolution experiments using molecular genetic mutation technologies and in vivo selection in recombinant bacterial strains.
ObjectiveAll technologies used for the experiments will be explained to the students in practice with the goal that they will be able to independently apply them for the course project and in future research endeavors. After the course, an individual report about the results obtained has to be prepared.
ContentThis class conducts and supports experiments for a specifically designed genuine research project. We will carry out biological-chemical enzyme evolution experiments using molecular genetic mutation technologies and in vivo selection in recombinant bacterial strains. The relevant technologies will be taught to the students, such as the preparation of competent cells, production and isolation of DNA fragments, transformation of gene libraries, and DNA sequencing. The course participants will generate a variety of different variants of a chorismate mutase. Individual enzyme catalysts will be purified and subsequently characterized using several different spectroscopic methods. The detailed chemical-physical analyses include determination of the enzymes' kinetic parameters, their molecular mass, and the integrity of the protein structure. The students will present the results obtained from their individual evolution experiments at the end of the semester. We expect that during this lab course we will not only generate novel enzymes, but also gain new mechanistic insights into the investigated catalyst.
Lecture notesThe necessary documents and protocols will be distributed to the participants during the course.
LiteratureGeneral literature to "Directed Evolution" and chorismate mutases, e.g.:

– Taylor, S. V., P. Kast & D. Hilvert. 2001. Investigating and engineering enzymes by genetic selection. Angew. Chem. Int. Ed. 40: 3310-3335.

– Jäckel, C., P. Kast & D. Hilvert. 2008. Protein design by directed evolution. Annu. Rev. Biophys. 37: 153-173.

– Roderer, K. & P. Kast. 2009. Evolutionary cycles for pericyclic reactions – Or why we keep mutating mutases. Chimia 63: 313-317.

Further literature will be indicated in the distributed script.
Prerequisites / Notice- This laboratory course will involve experiments that require a tight schedule and (sometimes) long (!) working days.
- The projects of this course are tightly linked to the ones of the Biology BSc course "529-0739-01 Biological Chemistry B: New Enzymes from Directed Evolution Experiments", which takes place as a block course during the month of November. There will be joint lectures for the participants of both courses during that time. The teaching language is English.
- The number of participants for the laboratory class is limited. It is mandatory to sign up for the course directly with P. Kast no later than September 1, prior to the start of the fall semester. Until then it will be decided whether the course will take place.
- A valid registration is considered a commitment for attendance of the entire semester course, as involved material orders and experimental preparations are necessary and, once the class has started, the flow of the experiments must not be interrupted by individual absences. In case of an emergency, please immediately notify P. Kast.
- For more information, see also http://www.kast.ethz.ch/teaching.html or contact P. Kast directly (HCI F 333, Tel. 044 632 29 08, kast@org.chem.ethz.ch).
529-0739-10LBiological Chemistry A: Technologies for Directed Evolution of Enzymes Restricted registration - show details
Advanced laboratory course or internship depending on lab course Biological Chemistry B

Candidates must inquire with P. Kast no later than September 1st whether course will take place (no self-enrollment)

Further information to registration and work hours: Link
13 credits16PP. A. Kast, D. Hilvert
AbstractDuring this semester course, methodologies will be taught for biological-chemical enzyme evolution experiments using molecular genetic mutation technologies and in vivo selection in recombinant bacterial strains.
ObjectiveAll technologies used for the experiments will be explained to the students in practice with the goal that they will be able to independently apply them for the course project and in future research endeavors. After the course, an individual report about the results obtained has to be prepared.
ContentThis class conducts and supports experiments for a specifically designed genuine research project. We will carry out biological-chemical enzyme evolution experiments using molecular genetic mutation technologies and in vivo selection in recombinant bacterial strains. The relevant technologies will be taught to the students, such as the preparation of competent cells, production and isolation of DNA fragments, transformation of gene libraries, and DNA sequencing. The course participants will generate a variety of different variants of a chorismate mutase. Individual enzyme catalysts will be purified and subsequently characterized using several different spectroscopic methods. The detailed chemical-physical analyses include determination of the enzymes' kinetic parameters, their molecular mass, and the integrity of the protein structure. The students will present the results obtained from their individual evolution experiments at the end of the semester. We expect that during this lab course we will not only generate novel enzymes, but also gain new mechanistic insights into the investigated catalyst.
Lecture notesThe necessary documents and protocols will be distributed to the participants during the course.
LiteratureGeneral literature to "Directed Evolution" and chorismate mutases, e.g.:

– Taylor, S. V., P. Kast & D. Hilvert. 2001. Investigating and engineering enzymes by genetic selection. Angew. Chem. Int. Ed. 40: 3310-3335.

– Jäckel, C., P. Kast & D. Hilvert. 2008. Protein design by directed evolution. Annu. Rev. Biophys. 37: 153-173.

– Roderer, K. & P. Kast. 2009. Evolutionary cycles for pericyclic reactions – Or why we keep mutating mutases. Chimia 63: 313-317.

Further literature will be indicated in the distributed script.
Prerequisites / Notice- This laboratory course will involve experiments that require a tight schedule and (sometimes) long (!) working days.
- The projects of this course are tightly linked to the ones of the Biology BSc course "529-0739-01 Biological Chemistry B: New Enzymes from Directed Evolution Experiments", which takes place as a block course during the month of November. There will be joint lectures for the participants of both courses during that time. The teaching language is English.
- The number of participants for the laboratory class is limited. It is mandatory to sign up for the course directly with P. Kast no later than September 1, prior to the start of the fall semester. Until then it will be decided whether the course will take place.
- A valid registration is considered a commitment for attendance of the entire semester course, as involved material orders and experimental preparations are necessary and, once the class has started, the flow of the experiments must not be interrupted by individual absences. In case of an emergency, please immediately notify P. Kast.
- For more information, see also http://www.kast.ethz.ch/teaching.html or contact P. Kast directly (HCI F 333, Tel. 044 632 29 08, kast@org.chem.ethz.ch).