Rudolf Glockshuber: Catalogue data in Autumn Semester 2016 |
Name | Prof. Dr. Rudolf Glockshuber |
Field | Molekularbiologie |
Address | Inst. f. Molekularbiol.u.Biophysik ETH Zürich, HPK E 17 Otto-Stern-Weg 5 8093 Zürich SWITZERLAND |
Telephone | +41 44 633 68 19 |
Fax | +41 44 633 10 36 |
rudi@mol.biol.ethz.ch | |
Department | Biology |
Relationship | Full Professor |
Number | Title | ECTS | Hours | Lecturers | |
---|---|---|---|---|---|
551-0013-AAL | Biochemistry Enrolment ONLY for MSc students with a decree declaring this course unit as an additional admission requirement. Any other students (e.g. incoming exchange students, doctoral students) CANNOT enrol for this course unit. | 2 credits | 4R | R. Glockshuber | |
Abstract | The lecture is a basic introductory course on the molecular principles of biology for students who need to pass this course for admission to their MSc curriculum. | ||||
Objective | The goal of this course is to give the students a basic understanding of the molecules that build a cell and make it function, and the basic principles of metabolism and molecular genetics | ||||
Content | The course content is based on the following chapters of the textbook Biochemistry (Berg, Tymoczko, Stryer, 7th edition, 2012, Freeman & Co, New York) Chapter 1: The molecular design of life Chapter 2: Protein composition and structure Chapter 3: Exploring proteins and proteomes Chapter 4: DNA, RNA and the flow of information Chapter 5: Exploring Genes and Genomes Chapter 7: Hemoglobin Chapter 8: Enzymes and the basic concepts of catalysis Chapter 11: Carbohydrates Chapter 12: Lipids and cell membranes Chapter 15: Metabolism: Basic concepts and design | ||||
Literature | Biochemistry (Berg, Tymoczko, Stryer, 7th edition, 2012, Freeman & Co, New York) | ||||
551-0015-00L | Biology I | 2 credits | 2V | R. Glockshuber, E. Hafen | |
Abstract | The lecture Biology I, together with the lecture Biology II in the following summer semester, is a basic, introductory course into Biology for Students of Materials Sciences and other students with biology as subsidiary subject. | ||||
Objective | The goal of this course is to give the students a basic understanding of the molecules that build a cell and make it function, and the basic principles of metabolism and molecular genetics. | ||||
Content | Die folgenden Kapitelnummern beziehen sich auf das der Vorlesung zugrundeliegende Lehrbuch "Biology" (Campbell & Rees, 10th edition, 2015) Kapitel 1-4 des Lehrbuchs werden als Grundwissen vorausgesetzt 1. Aufbau der Zelle Kapitel 5: Struktur und Funktion biologischer Makromoleküle Kapitel 6: Eine Tour durch die Zelle Kaptiel 7: Membranstruktur und-funktion Kapitel 8: Einführung in den Stoffwechsel Kapitel 9: Zelluläre Atmung und Speicherung chemischer Energie Kapitel 10: Photosynthese Kapitel 12: Der Zellzyklus Kapitel 17: Vom Gen zum Protein 2. Allgemeine Genetik Kapitel 13: Meiose und Reproduktionszyklen Kapitel 14: Mendel'sche Genetik Kapitel 15: Die chromosomale Basis der Vererbung Kapitel 16: Die molekulare Grundlage der Vererbung Kapitel 18: Genetik von Bakterien und Viren Kapitel 46: Tierische Reproduktion Grundlagen des Stoffwechsels und eines Überblicks über molekulare Genetik | ||||
Lecture notes | Der Vorlesungsstoff ist sehr nahe am Lehrbuch gehalten, Skripte werden ggf. durch die Dozenten zur Verfügung gestellt. | ||||
Literature | Das folgende Lehrbuch ist Grundlage für die Vorlesungen Biologie I und II: „Biology“, Campbell and Rees, 10th Edition, 2015, Pearson/Benjamin Cummings, ISBN 978-3-8632-6725-4 | ||||
Prerequisites / Notice | Zur Vorlesung Biologie I gibt es während der Prüfungssessionen eine einstündige, schriftliche Prüfung. Die Vorlesung Biologie II wird separat geprüft. | ||||
551-0307-00L | Molecular and Structural Biology I: Protein Structure and Function D-BIOL BSc students are obliged to take part I and part II (next semester) as a two-semester course | 3 credits | 2V | R. Glockshuber, K. Locher, E. Weber-Ban | |
Abstract | Biophysics of protein folding, membrane proteins and biophysics of membranes, enzymatic catalysis, catalytic RNA and RNAi, current topics in protein biophysics and structural biology. | ||||
Objective | Understanding of structure-function relationships in proteins and in protein folding, detailed understanding of biophysics and physical methods as well as modern methods for protein purification and microanalytics. | ||||
Lecture notes | Scripts on the individual topics can be found under http://www.mol.biol.ethz.ch/teaching. | ||||
Literature | Basics: - Creighton, T.E., Proteins, Freeman, (1993) - Fersht, A., Enzyme, Structure and Mechanism in Protein Science (1999), Freeman. - Berg, Tymoczko, Stryer: Biochemistry (5th edition), Freeman (2001). Current topics: References will be given during the lectures. . | ||||
551-0438-00L | Protein Folding, Assembly and Degradation Number of participants limited to 6. | 6 credits | 7G | R. Glockshuber, E. Weber-Ban | |
Abstract | Students will carry out defined research projects related to the current research topics of the groups of Prof. Glockshuber and Prof. Weber-Ban. The topics include mechanistic studies on the assembly of adhesive pili from pathogenic bacteria, disulfide bond formation in the bacterial periplasm, ATP-dependent chaperone-protease complexes and formation of amyloid deposits in Alzheimer's disese. | ||||
Objective | The course should enable the students to understand and apply biophysical methods, in particular kinetic and spectroscopic methods, to unravel the mechanism of complex reactions of biological macromolecules and assemblies in a quantitative manner. | ||||
Content | The students will be tutored in their experimental work by doctoral or postdoctoral students from the Glockshuber or Weber-Ban group. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as excercises on the numeric evaluation of biophysical data, and literature work. Participation in one of the following projects will be possible: Projects of the Glockshuber group: - Purification, biophysical characterization and structure determiation of enzymes required for disulfide bond formation in the periplasm of Gram-negative bacteria. - Mechanistic studies on the assembly of type 1 pili from pathogenic Escherichia coli strains. In vitro reconstitution of pilus assembly from all purified components. Characterization of folding, stability and assembly behaviour of individual pilus subunits. - Identification of intermediates in the aggregation of the human Abeta peptide Experimental work on these projects involves - Molecular cloning, recombinant protein production in E. coli and protein purification - Protein crystallization - Thermodynamic and kinetic characterization of conformational changes in proteins and protein-ligand interactions by fluorescence and circular dischoism spectroscopy - Analysis of rapid reactions by stopped-flow fluorescence - Negative-stain electron microscopy - Light scattering Projects of the Weber-Ban group: - Generation and purification of site-directed variants of the E. coli ClpA/P protease and chaperone-proteasome complexes from other organisms, their biophysical characterization, including rapid kinetics by stopped-flow methods, ATPase activity measurtements, negative-stain electron microscopy and light scattering | ||||
Lecture notes | No script | ||||
Literature | Literature related to the individual projects will be provided on the first day of the course. | ||||
Prerequisites / Notice | Attendance of the concept course "Biomolecular Structure and Mechanism I: Protein Structure and Function" (551-0307-00L) in the autumn semester is highly recommended for acquiring the theoretical background to this block course. | ||||
551-1323-AAL | Fundamentals of Biology II: Biochemistry and Molecular Biology Enrolment ONLY for MSc students with a decree declaring this course unit as an additional admission requirement. Any other students (e.g. incoming exchange students, doctoral students) CANNOT enrol for this course unit. | 4 credits | 11R | K. Locher, N. Ban, R. Glockshuber, E. Weber-Ban | |
Abstract | The course provides an introduction to Biochemistry / Molecular Biology with some emphasis on chemical and biophysical aspects. | ||||
Objective | Topics include the structure-function relationship of proteins / nucleic acids, protein folding, enzymatic catalysis, cellular pathways involved in bioenergetics and the biosynthesis and breakdown of amino acids, glycans, nucleotides, fatty acids and phospholipids, and steroids. There will also be a discussion of DNA replication and repair, transcription, and translation. | ||||
Lecture notes | none | ||||
Literature | "Biochemistry", Berg/Tymoczko/Stryer, 8th edition, Palgrave Macmillan, International edition | ||||
551-1323-00L | Fundamentals of Biology II: Biochemistry and Molecular Biology | 4 credits | 4V | K. Locher, N. Ban, R. Glockshuber, E. Weber-Ban | |
Abstract | The course provides an introduction to Biochemistry / Molecular Biology with some emphasis on chemical and biophysical aspects. | ||||
Objective | Topics include the structure-function relationship of proteins / nucleic acids, protein folding, enzymatic catalysis, cellular pathways involved in bioenergetics and the biosynthesis and breakdown of amino acids, glycans, nucleotides, fatty acids and phospholipids, and steroids. There will also be a discussion of DNA replication and repair, transcription, and translation. | ||||
Lecture notes | none | ||||
Literature | mandatory: "Biochemistry", Autoren: Berg/Tymoczko/Stryer, 8th edition, Palgrave Macmillan, International edition (the English version will be preordered at the Polybuchhandlung) | ||||
Prerequisites / Notice | Some of the lectures are given in the English language. | ||||
551-1411-00L | Molecular and Structural Biology III: Current Topics The course will only take place with a minimum of 6 participants. | 2 credits | 1V | K. Locher, F. Allain, N. Ban, R. Glockshuber, M. Pilhofer, E. Weber-Ban | |
Abstract | The course discusses current topics and cutting edge research in the structural, molecular, and biochemical study of cellular macromolecules. Student participation is an essential component of the course and will contribute to the exam grade | ||||
Objective | The goal is to discuss cutting edge research in the structural, molecular, and biochemical study of cellular macromolecules. Students will also have an opportunity to present and discuss recent breakthroughs relevant to the research fields presented by the faculty teaching the course (see http://www.mol.biol.ethz.ch/research.html for details on the topics). | ||||
Literature | Will be distributed by the instructors | ||||
551-1619-00L | Structural Biology | 1 credit | 1K | R. Glockshuber, F. Allain, N. Ban, K. Locher, E. Weber-Ban, G. Wider, K. Wüthrich | |
Abstract | The course consists of a series of research seminars on Structural Biology, Biochemistry and Biophysics, given by both scientists of the National Center of Competence in Research (NCCR) in Structural Biology and external speakers. Information on the individual seminars is provided on the following websites: http://www.structuralbiology.uzh.ch/educ002.asp http://www.biol.ethz.ch/dbiol-cal/index | ||||
Objective | The goal of this course is to provide doctoral and postdoctoral students with a broad overview on the most recent developments in biochemistry, structural biology and biophysics. |