551-0362-00L  Analysis of Signaling Networks by Mass Spectrometry

SemesterSpring Semester 2019
LecturersM. Gstaiger, M. Claassen, B. Wollscheid
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 10.

The enrolment is done by the D-BIOL study administration.

AbstractThis course provides the theoretical and practical basis for the biochemical and computational analysis of signaling networks using quantitative mass spectrometry and advanced statistical methods.
ObjectiveIn this course we will introduce basic and emerging techniques to study dynamic signalling networks using state of the art quantitative mass spectrometry techniques. This will involve the systematic characterization of signaling networks by affinity purification and phospho-peptide enrichment combined with quantitative mass spectrometry. We will also introduce and apply computational tools for statistical analysis, data visualization and network inference to build new hypothesis on the basis of the obtained data.
Prerequisites / NoticeThis course requires a basic knowledge in mass spectrometry based proteomics and experience in computational data processing using R or MatLab. Ideally this course should be combined with course 551-0352-00L "Protein Analysis by Mass Spectrometry".